Useful agents for the study of glutathione metabolism in erythrocytes

organic hydroperoxides

Satish Srivastava, Y. C. Awasthi, E. Beutler

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

t Butyl hydroperoxide and cumene hydroperoxide, both known to be substrates for glutathione peroxidase, were used to oxidize erythrocyte GSH. Addition of concentrations of hydroperoxides equimolar with respect to GSH in the erythrocytes or whole blood quantitatively oxidizes GSH in the erythrocytes with a half time of 4.5 sec at 37°C and about three times as long at 4°C. In the presence of glucose, normal erythrocytes regenerate all the GSH in about 25 min. However, glucose 6 phosphate dehydrogenase deficient erythrocytes failed to regenerate GSH. Treatment of erythrocytes with hydroperoxides does not affect erythrocyte survival in rabbits. Oxidation of erythrocyte GSH with equimolar concentrations of hydroperoxides does not lead to formation of mixed disulphides of haemoglobin and GSH. The hydroperoxides do not affect erythrocyte sulphides of haemoglobin and GSH. The hydroperoxides do not affect erythrocyte glycolytic and hexose monophosphate shunt pathway enzymes. Previous studies on transport of GSSG from erythrocytes were confirmed by using t butyl hydroperoxide to oxidize erythrocyte GSH.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalBiochemical Journal
Volume139
Issue number2
StatePublished - 1974
Externally publishedYes

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Metabolism
Hydrogen Peroxide
Glutathione
Erythrocytes
tert-Butylhydroperoxide
Hemoglobins
Hexoses
Glutathione Disulfide
Glucosephosphate Dehydrogenase
Sulfides
Glutathione Peroxidase
Disulfides
Blood
Erythrocyte Aging
Glucose
Oxidation
Pentose Phosphate Pathway
Substrates
Enzymes
Rabbits

ASJC Scopus subject areas

  • Biochemistry

Cite this

Srivastava, S., Awasthi, Y. C., & Beutler, E. (1974). Useful agents for the study of glutathione metabolism in erythrocytes: organic hydroperoxides. Biochemical Journal, 139(2), 289-295.

Useful agents for the study of glutathione metabolism in erythrocytes : organic hydroperoxides. / Srivastava, Satish; Awasthi, Y. C.; Beutler, E.

In: Biochemical Journal, Vol. 139, No. 2, 1974, p. 289-295.

Research output: Contribution to journalArticle

Srivastava, S, Awasthi, YC & Beutler, E 1974, 'Useful agents for the study of glutathione metabolism in erythrocytes: organic hydroperoxides', Biochemical Journal, vol. 139, no. 2, pp. 289-295.
Srivastava, Satish ; Awasthi, Y. C. ; Beutler, E. / Useful agents for the study of glutathione metabolism in erythrocytes : organic hydroperoxides. In: Biochemical Journal. 1974 ; Vol. 139, No. 2. pp. 289-295.
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