Using DNA barcoding to identify species within euphorbiaceae

Xiaohui Pang, Jingyuan Song, Yingjie Zhu, Caixiang Xie, Shilin Chen

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified >90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.

Original languageEnglish (US)
Pages (from-to)1784-1786
Number of pages3
JournalPlanta Medica
Volume76
Issue number15
DOIs
StatePublished - Nov 2 2010
Externally publishedYes

Keywords

  • DNA barcoding
  • Euphorbiaceae
  • intra/interspecific genetic divergence
  • species identification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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  • Cite this

    Pang, X., Song, J., Zhu, Y., Xie, C., & Chen, S. (2010). Using DNA barcoding to identify species within euphorbiaceae. Planta Medica, 76(15), 1784-1786. https://doi.org/10.1055/s-0030-1249806