Using immunoproteomics to identify alpha-enolase as an autoantigen in liver fibrosis

Bo Peng, Xueyong Huang, Ernesto S. Nakayasu, John R. Petersen, Suimin Qiu, Igor C. Almeida, Jian Ying Zhang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Liver fibrosis results from extracellular matrix accumulation during the wound healing process when the liver is insulted with chronic viral infection, inflammation, or alcoholic diseases. The current diagnosis of liver fibrosis is mainly dependent on biopsy, which is an invasive approach. Identification of serological biomarkers has been considered as the most promising way for early detection of the disease. Although several biomarkers in liver fibrosis have been identified, the problem is that these markers can be also detected in fibrogenesis that occurred in other organs. In this study, we have identified and characterized some cellular proteins that can be recognized by autoantibodies in the sera from patients with precirrhotic stage of liver fibrosis. Among 180 sera from patients with liver fibrosis, 14.4% (26/180) of sera contained autoantibody against a protein migrating around 47 kDa on SDS-PAGE gel. Indirect immunofluorescence assay using purified autoantibody against the 47-kDa protein showed that this protein mainly localized in the cytoplasm. Using immunoproteomic approach, the 47-kDa protein was identified as alpha-enolase. In further study, the frequency of antialpha-enolase antibody in sera from patients with precirrhotic stage of liver fibrosis (21.6%, 27/125) was significantly higher than that in sera from patients with cirrhosis (9.1%, 5/55) and liver cancer (14.3%, 12/84), as well as in sera from healthy individuals (4.1%, 3/74). Therefore, alpha-enolase is an autoantigen that elicits autoimmune response in liver fibrosis and can be a potential prognostic factor for liver fibrosis diagnosis.

Original languageEnglish (US)
Pages (from-to)1789-1796
Number of pages8
JournalJournal of Proteome Research
Volume12
Issue number4
DOIs
StatePublished - Apr 5 2013

Fingerprint

Phosphopyruvate Hydratase
Autoantigens
Liver Cirrhosis
Liver
Serum
Autoantibodies
Proteins
Biomarkers
Virus Diseases
Liver Neoplasms
Indirect Fluorescent Antibody Technique
Autoimmunity
Wound Healing
Biopsy
Extracellular Matrix
Polyacrylamide Gel Electrophoresis
Early Diagnosis
Cytoplasm
Fibrosis
Gels

Keywords

  • alpha-enolase
  • autoantigen
  • autoimmune response
  • immunoproteomics
  • liver fibrosis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Peng, B., Huang, X., Nakayasu, E. S., Petersen, J. R., Qiu, S., Almeida, I. C., & Zhang, J. Y. (2013). Using immunoproteomics to identify alpha-enolase as an autoantigen in liver fibrosis. Journal of Proteome Research, 12(4), 1789-1796. https://doi.org/10.1021/pr3011342

Using immunoproteomics to identify alpha-enolase as an autoantigen in liver fibrosis. / Peng, Bo; Huang, Xueyong; Nakayasu, Ernesto S.; Petersen, John R.; Qiu, Suimin; Almeida, Igor C.; Zhang, Jian Ying.

In: Journal of Proteome Research, Vol. 12, No. 4, 05.04.2013, p. 1789-1796.

Research output: Contribution to journalArticle

Peng, B, Huang, X, Nakayasu, ES, Petersen, JR, Qiu, S, Almeida, IC & Zhang, JY 2013, 'Using immunoproteomics to identify alpha-enolase as an autoantigen in liver fibrosis', Journal of Proteome Research, vol. 12, no. 4, pp. 1789-1796. https://doi.org/10.1021/pr3011342
Peng, Bo ; Huang, Xueyong ; Nakayasu, Ernesto S. ; Petersen, John R. ; Qiu, Suimin ; Almeida, Igor C. ; Zhang, Jian Ying. / Using immunoproteomics to identify alpha-enolase as an autoantigen in liver fibrosis. In: Journal of Proteome Research. 2013 ; Vol. 12, No. 4. pp. 1789-1796.
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