Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals

Fatalmoudou Tandina, Sirama Niaré, Maureen Laroche, Abdoulaye K. Koné, Adama Z. Diarra, Abdoulaye Ongoiba, Jean Michel Berenger, Ogobara K. Doumbo, Didier Raoult, Philippe Parola

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.

Original languageEnglish (US)
Pages (from-to)1170-1182
Number of pages13
JournalVictorian Literature and Culture
Volume145
Issue number9
DOIs
StatePublished - Aug 1 2018
Externally publishedYes

Keywords

  • MALDI-TOF MS
  • Mali
  • Whatman
  • blood meals
  • field
  • mosquito

ASJC Scopus subject areas

  • Cultural Studies
  • Literature and Literary Theory

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