Utility of CE-MS data in protein identification

Brad J. Williams, William K. Russell, David H. Russell

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A new method for displaying CE-MALDI-MS data for proteolytic digests is described. This data display mode yields distinct charge-based trends for plots of mass-to-charge (m/z) versus CE migration time. These trends arise owing to the in-solution charge state of the peptides, and this interpretation was confirmed by using empirical peptide electromigration models and peptide standards as charge-state markers. These charge-state specific trends exhibit analytical utility by providing additional chemical information about the peptides, which increases the confidence level of peptide identification and provides a rapid means for screening for posttranslationally modified peptides.

Original languageEnglish (US)
Pages (from-to)3850-3855
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number10
DOIs
StatePublished - May 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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