Utility of CE-MS data in protein identification

Brad J. Williams, William Russell, David H. Russell

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A new method for displaying CE-MALDI-MS data for proteolytic digests is described. This data display mode yields distinct charge-based trends for plots of mass-to-charge (m/z) versus CE migration time. These trends arise owing to the in-solution charge state of the peptides, and this interpretation was confirmed by using empirical peptide electromigration models and peptide standards as charge-state markers. These charge-state specific trends exhibit analytical utility by providing additional chemical information about the peptides, which increases the confidence level of peptide identification and provides a rapid means for screening for posttranslationally modified peptides.

Original languageEnglish (US)
Pages (from-to)3850-3855
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number10
DOIs
StatePublished - May 15 2007
Externally publishedYes

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Peptides
Proteins
Electromigration
Screening
Display devices

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Utility of CE-MS data in protein identification. / Williams, Brad J.; Russell, William; Russell, David H.

In: Analytical Chemistry, Vol. 79, No. 10, 15.05.2007, p. 3850-3855.

Research output: Contribution to journalArticle

Williams, Brad J. ; Russell, William ; Russell, David H. / Utility of CE-MS data in protein identification. In: Analytical Chemistry. 2007 ; Vol. 79, No. 10. pp. 3850-3855.
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