Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection

Jesse H. Erasmus, James Needham, Syamal Raychaudhuri, Michael S. Diamond, David Beasley, Stan Morkowski, Henrik Salje, Ildefonso Fernandez Salas, Dal Young Kim, Ilya Frolov, Farooq Nasar, Scott Weaver

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In December of 2013, chikungunya virus (CHIKV), an alphavirus in the family Togaviridae, was introduced to the island of Saint Martin in the Caribbean, resulting in the first autochthonous cases reported in the Americas. As of January 2015, local and imported CHIKV has been reported in 50 American countries with over 1.1 million suspected cases. CHIKV causes a severe arthralgic disease for which there are no approved vaccines or therapeutics. Furthermore, the lack of a commercially available, sensitive, and affordable diagnostic assay limits surveillance and control efforts. To address this issue, we utilized an insect-specific alphavirus, Eilat virus (EILV), to develop a diagnostic antigen that does not require biosafety containment facilities to produce. We demonstrated that EILV/CHIKV replicates to high titers in insect cells and can be applied directly in enzyme-linked immunosorbent assays without inactivation, resulting in highly sensitive detection of recent and past CHIKV infection, and outperforming traditional antigen preparations.

Original languageEnglish (US)
Article numbere0004119
JournalPLoS Neglected Tropical Diseases
Volume9
Issue number10
DOIs
StatePublished - Oct 22 2015

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Chikungunya virus
Alphavirus
Viruses
Infection
Insects
Togaviridae
Antigens
Islands
Vaccines
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection. / Erasmus, Jesse H.; Needham, James; Raychaudhuri, Syamal; Diamond, Michael S.; Beasley, David; Morkowski, Stan; Salje, Henrik; Fernandez Salas, Ildefonso; Kim, Dal Young; Frolov, Ilya; Nasar, Farooq; Weaver, Scott.

In: PLoS Neglected Tropical Diseases, Vol. 9, No. 10, e0004119, 22.10.2015.

Research output: Contribution to journalArticle

Erasmus, JH, Needham, J, Raychaudhuri, S, Diamond, MS, Beasley, D, Morkowski, S, Salje, H, Fernandez Salas, I, Kim, DY, Frolov, I, Nasar, F & Weaver, S 2015, 'Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection', PLoS Neglected Tropical Diseases, vol. 9, no. 10, e0004119. https://doi.org/10.1371/journal.pntd.0004119
Erasmus, Jesse H. ; Needham, James ; Raychaudhuri, Syamal ; Diamond, Michael S. ; Beasley, David ; Morkowski, Stan ; Salje, Henrik ; Fernandez Salas, Ildefonso ; Kim, Dal Young ; Frolov, Ilya ; Nasar, Farooq ; Weaver, Scott. / Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection. In: PLoS Neglected Tropical Diseases. 2015 ; Vol. 9, No. 10.
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