v-mos protein produced by in vitro translation has protein kinase activity

Norbert K. Herzog, Michael Nash, Louis S. Ramagli, Ralph B. Arlinghaus

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The v-mos protein, termed p37v-mos, has a closely associated serine/threonine protein kinase activity. To provide further information about its protein kinase activity, we tested the activity of p37v-mos produced in a cell-free translation system from transcripts generated from a cloned v-mos gene. Anti-mos(37-55) immunoprecipitates of in vitro-produced p37v-mos were found to possess serine/threonine protein kinase activity, whereas those obtained with anti-mos(260-271), known to block v-mos autophosphorylation, lacked kinase activity. The phosphorylated products were identical in size to p37v-mos and p43v-mos produced in protein kinase assays from Moloney murine sarcoma virus-infected cells expressing authentic p37v-mos. These results provide further proof that the protein kinase activity associated with p37v-mos is an intrinsic property of the v-mos gene product. This translation system also provides a useful experimental model to study the activation of the mos protein kinase. Thus, protein kinase assays performed on [35S]methionine-labeled p37v-mos produced p43v-mos at the expense of p37v-mos. Phosphatase treatment removed the p43v-mos species, resulting in an increase of the p37v-mos-sized protein, confirming our previous interpretation that p43v-mos is a hyperphosphorylated form of p37v-mos.

Original languageEnglish (US)
Pages (from-to)3093-3096
Number of pages4
JournalJournal of Virology
Volume64
Issue number6
StatePublished - 1990

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Oncogene Proteins v-mos
protein kinases
Protein Kinases
translation (genetics)
mos Genes
Protein-Serine-Threonine Kinases
proteins
Moloney murine sarcoma virus
Cell-Free System
protein phosphorylation
assays
Phosphoric Monoester Hydrolases
Methionine
methionine
phosphotransferases (kinases)
Theoretical Models
Phosphotransferases
genes
In Vitro Techniques
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Herzog, N. K., Nash, M., Ramagli, L. S., & Arlinghaus, R. B. (1990). v-mos protein produced by in vitro translation has protein kinase activity. Journal of Virology, 64(6), 3093-3096.

v-mos protein produced by in vitro translation has protein kinase activity. / Herzog, Norbert K.; Nash, Michael; Ramagli, Louis S.; Arlinghaus, Ralph B.

In: Journal of Virology, Vol. 64, No. 6, 1990, p. 3093-3096.

Research output: Contribution to journalArticle

Herzog, NK, Nash, M, Ramagli, LS & Arlinghaus, RB 1990, 'v-mos protein produced by in vitro translation has protein kinase activity', Journal of Virology, vol. 64, no. 6, pp. 3093-3096.
Herzog NK, Nash M, Ramagli LS, Arlinghaus RB. v-mos protein produced by in vitro translation has protein kinase activity. Journal of Virology. 1990;64(6):3093-3096.
Herzog, Norbert K. ; Nash, Michael ; Ramagli, Louis S. ; Arlinghaus, Ralph B. / v-mos protein produced by in vitro translation has protein kinase activity. In: Journal of Virology. 1990 ; Vol. 64, No. 6. pp. 3093-3096.
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