Vaccine Potential of Nipah Virus-Like Particles

Pramila Walpita, Jennifer Barr, Michael Sherman, Christopher F. Basler, Linfa Wang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Nipah virus (NiV) was first recognized in 1998 in a zoonotic disease outbreak associated with highly lethal febrile encephalitis in humans and a predominantly respiratory disease in pigs. Periodic deadly outbreaks, documentation of person-to-person transmission, and the potential of this virus as an agent of agroterror reinforce the need for effective means of therapy and prevention. In this report, we describe the vaccine potential of NiV virus-like particles (NiV VLPs) composed of three NiV proteins G, F and M. Co-expression of these proteins under optimized conditions resulted in quantifiable amounts of VLPs with many virus-like/vaccine desirable properties including some not previously described for VLPs of any paramyxovirus: The particles were fusogenic, inducing syncytia formation; PCR array analysis showed NiV VLP-induced activation of innate immune defense pathways; the surface structure of NiV VLPs imaged by cryoelectron microscopy was dense, ordered, and repetitive, and consistent with similarly derived structure of paramyxovirus measles virus. The VLPs were composed of all the three viral proteins as designed, and their intracellular processing also appeared similar to NiV virions. The size, morphology and surface composition of the VLPs were consistent with the parental virus, and importantly, they retained their antigenic potential. Finally, these particles, formulated without adjuvant, were able to induce neutralizing antibody response in Balb/c mice. These findings indicate vaccine potential of these particles and will be the basis for undertaking future protective efficacy studies in animal models of NiV disease.

Original languageEnglish (US)
Article numbere18437
JournalPLoS One
Volume6
Issue number4
DOIs
StatePublished - 2011

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Nipah Virus
Nipah virus
virus-like particles
Viruses
Virion
Vaccines
vaccines
Respirovirus
Disease Outbreaks
viruses
Cryoelectron Microscopy
Measles virus
Zoonoses
Viral Proteins
Virus Diseases
Encephalitis
Giant Cells
Neutralizing Antibodies
viral proteins
Surface structure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Walpita, P., Barr, J., Sherman, M., Basler, C. F., & Wang, L. (2011). Vaccine Potential of Nipah Virus-Like Particles. PLoS One, 6(4), [e18437]. https://doi.org/10.1371/journal.pone.0018437

Vaccine Potential of Nipah Virus-Like Particles. / Walpita, Pramila; Barr, Jennifer; Sherman, Michael; Basler, Christopher F.; Wang, Linfa.

In: PLoS One, Vol. 6, No. 4, e18437, 2011.

Research output: Contribution to journalArticle

Walpita, P, Barr, J, Sherman, M, Basler, CF & Wang, L 2011, 'Vaccine Potential of Nipah Virus-Like Particles', PLoS One, vol. 6, no. 4, e18437. https://doi.org/10.1371/journal.pone.0018437
Walpita, Pramila ; Barr, Jennifer ; Sherman, Michael ; Basler, Christopher F. ; Wang, Linfa. / Vaccine Potential of Nipah Virus-Like Particles. In: PLoS One. 2011 ; Vol. 6, No. 4.
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