Vacuolar-type H+-ATPases at the plasma membrane regulate pH and cell migration in microvascular endothelial cells

J. D. Rojas, S. R. Sennoune, D. Maiti, K. Bakunts, M. Reuveni, S. C. Sanka, G. M. Martinez, E. A. Seftor, C. J. Meininger, G. Wu, D. E. Wesson, M. J.C. Hendrix, R. Martínez-Zaguilán

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Microvascular endothelial cells involved in angiogenesis are exposed to an acidic environment that is not conducive for growth and survival. These cells must exhibit a dynamic intracellular (cytosolic) pH (pHcyt) regulatory mechanism to cope with acidosis, in addition to the ubiquitous Na+/H+ exchanger and HCO3--based H+-transporting systems. We hypothesize that the presence of plasmalemmal vacuolar-type proton ATPases (pmV-ATPases) allows microvascular endothelial cells to better cope with this acidic environment and that pmV-ATPases are required for cell migration. This study indicates that microvascular endothelial cells, which are more migratory than macrovascular endothelial cells, express pmV-ATPases. Spectral imaging microscopy indicates a more alkaline pHcyt at the leading than at the lagging edge of microvascular endothelial cells. Treatment of microvascular endothelial cells with V-ATPase inhibitors decreases the proton fluxes via pmV-ATPases and cell migration. These data suggest that pmV-ATPases are essential for pH cyt regulation and cell migration in microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)H1147-H1157
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume291
Issue number3
DOIs
StatePublished - Sep 13 2006
Externally publishedYes

Keywords

  • Bafilomycin
  • Buffering capacity
  • Carboxyseminaphthorhodafluor-1
  • Fluorescence spectroscopy
  • Macrovascular endothelial cells
  • Migration
  • Proton fluxes
  • Sodium/hydrogen exchanger

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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    Rojas, J. D., Sennoune, S. R., Maiti, D., Bakunts, K., Reuveni, M., Sanka, S. C., Martinez, G. M., Seftor, E. A., Meininger, C. J., Wu, G., Wesson, D. E., Hendrix, M. J. C., & Martínez-Zaguilán, R. (2006). Vacuolar-type H+-ATPases at the plasma membrane regulate pH and cell migration in microvascular endothelial cells. American Journal of Physiology - Heart and Circulatory Physiology, 291(3), H1147-H1157. https://doi.org/10.1152/ajpheart.00166.2006