Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2

Ruby Tiwari, Surendra S. Negi, Benjamin Braun, Werner Braun, Anna Pomés, Martin D. Chapman, Randall M. Goldblum, Terumi Midoro-Horiuti

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Bla g 2, one of the major cockroach allergens, induces a strong IgE response against conformational epitopes, and on reexposure, sensitized individuals often display symptoms of allergic rhinitis and asthma. The aim of the current study was to perform a test of the efficacy of a modified phage display screening, characterization of selected phages and an automated algorithm, EpiSearch, in locating an important conformational epitope. Methods: The monoclonal antibody 7C11, which partially inhibits the binding of patient IgE antibodies to Bla g 2, was used to screen a random peptide phage library. After 3 rounds of panning, 32 phage clones were isolated and the amino acid sequences of their peptides were determined. The relative affinity and specificity of the binding of these peptides to 7C11 were tested in ELISAs. The amino acid composition of these peptides was then matched with clusters of residues on the surface of the 3-dimensional (3D) structure of Bla g 2, using our EpiSearch algorithm. Results: The amino acid sequences of the peptides on selected phages differed at only one position, occupied by 1 of 2 negatively charged residues. The two 12-mer sequences bound to 7C11 with similar avidity and specificity. There was good concordance between the residues in the 3D clusters identified from our phage display/computational method with the co-crystal structural analysis. Conclusion: Conformational epitopes may be mapped through screening of clones from random peptide phage display libraries and EpiSearch.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume157
Issue number4
DOIs
StatePublished - Mar 2012

Fingerprint

Bacteriophages
Epitopes
Peptide Library
Peptides
Immunoglobulin E
Amino Acid Sequence
Clone Cells
Cockroaches
Allergens
Asthma
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Amino Acids
Antibodies

Keywords

  • Bla g 2
  • Cockroach allergen
  • Conformational epitope
  • EpiSearch
  • Monoclonal antibody
  • Random peptide phage display library

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2. / Tiwari, Ruby; Negi, Surendra S.; Braun, Benjamin; Braun, Werner; Pomés, Anna; Chapman, Martin D.; Goldblum, Randall M.; Midoro-Horiuti, Terumi.

In: International Archives of Allergy and Immunology, Vol. 157, No. 4, 03.2012, p. 323-330.

Research output: Contribution to journalArticle

Tiwari, Ruby ; Negi, Surendra S. ; Braun, Benjamin ; Braun, Werner ; Pomés, Anna ; Chapman, Martin D. ; Goldblum, Randall M. ; Midoro-Horiuti, Terumi. / Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2. In: International Archives of Allergy and Immunology. 2012 ; Vol. 157, No. 4. pp. 323-330.
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