Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species

Shilin Chen, Hui Yao, Jianping Han, Chang Liu, Jingyuan Song, Linchun Shi, Yingjie Zhu, Xinye Ma, Ting Gao, Xiaohui Pang, Kun Luo, Ying Li, Xiwen Li, Xiaocheng Jia, Yulin Lin, Christine Leon

Research output: Contribution to journalArticle

766 Citations (Scopus)

Abstract

Background: The plant working group of the Consortium for the Barcode of Life recommended the two-locus combination of rbcL + matK as the plant barcode, yet the combination was shown to successfully discriminate among 907 samples from 550 species at the species level with a probability of 72%. The group admits that the two-locus barcode is far from perfect due to the low identification rate, and the search is not over. Methodology/Principal Findings: Here, we compared seven candidate DNA barcodes (psbA-trnH, matK, rbcL, rpoC1, ycf5, ITS2, and ITS) from medicinal plant species. Our ranking criteria included PCR amplification efficiency, differential intra- and inter-specific divergences, and the DNA barcoding gap. Our data suggest that the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA represents the most suitable region for DNA barcoding applications. Furthermore, we tested the discrimination ability of ITS2 in more than 6600 plant samples belonging to 4800 species from 753 distinct genera and found that the rate of successful identification with the ITS2 was 92.7% at the species level. Conclusions: The ITS2 region can be potentially used as a standard DNA barcode to identify medicinal plants and their closely related species. We also propose that ITS2 can serve as a novel universal barcode for the identification of a broader range of plant taxa.

Original languageEnglish (US)
Article numbere8613
JournalPLoS One
Volume5
Issue number1
DOIs
StatePublished - Jan 7 2010
Externally publishedYes

Fingerprint

barcoding
DNA barcoding
Medicinal Plants
medicinal plants
DNA
loci
Ribosomal DNA
ribosomal DNA
internal transcribed spacers
Amplification
sampling
Polymerase Chain Reaction
methodology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chen, S., Yao, H., Han, J., Liu, C., Song, J., Shi, L., ... Leon, C. (2010). Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One, 5(1), [e8613]. https://doi.org/10.1371/journal.pone.0008613

Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. / Chen, Shilin; Yao, Hui; Han, Jianping; Liu, Chang; Song, Jingyuan; Shi, Linchun; Zhu, Yingjie; Ma, Xinye; Gao, Ting; Pang, Xiaohui; Luo, Kun; Li, Ying; Li, Xiwen; Jia, Xiaocheng; Lin, Yulin; Leon, Christine.

In: PLoS One, Vol. 5, No. 1, e8613, 07.01.2010.

Research output: Contribution to journalArticle

Chen, S, Yao, H, Han, J, Liu, C, Song, J, Shi, L, Zhu, Y, Ma, X, Gao, T, Pang, X, Luo, K, Li, Y, Li, X, Jia, X, Lin, Y & Leon, C 2010, 'Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species', PLoS One, vol. 5, no. 1, e8613. https://doi.org/10.1371/journal.pone.0008613
Chen, Shilin ; Yao, Hui ; Han, Jianping ; Liu, Chang ; Song, Jingyuan ; Shi, Linchun ; Zhu, Yingjie ; Ma, Xinye ; Gao, Ting ; Pang, Xiaohui ; Luo, Kun ; Li, Ying ; Li, Xiwen ; Jia, Xiaocheng ; Lin, Yulin ; Leon, Christine. / Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. In: PLoS One. 2010 ; Vol. 5, No. 1.
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