TY - JOUR
T1 - Validity of abbreviated oral fat tolerance tests for assessing postprandial lipemia
AU - Maraki, Maria
AU - Aggelopoulou, Niki
AU - Christodoulou, Nektarios
AU - Katsarou, Christina
AU - Anapliotis, Panagiotis
AU - Kavouras, Stavros A.
AU - Panagiotakos, Demosthenes
AU - Sidossis, Labros S.
N1 - Funding Information:
MM was supported by the Greek Governmental Institute of Scholarships. This study was partially supported by the Department of Internal Medicine and the Sealy Center on Aging, University of Texas Medical Branch at Galveston, the Department of Nutrition and Dietetics Graduate Program, Harokopio University of Athens and the Hellenic Heart Foundation, Athens Greece.
PY - 2011/12
Y1 - 2011/12
N2 - Background & aims: Postprandial lipemia is assessed using the oral fat tolerance test (OFTT), a six-hour procedure requiring hourly blood sampling. In order to simplify the test, we investigated whether a) a single postprandial triacylglycerol concentration, b) an OFTT lasting less than six hours or c) an OFTT requiring fewer blood samples, may accurately assess postprandial lipemia under various conditions. Methods: Seventy-two subjects underwent a conventional OFTT. Predictability of single-point concentrations and time-shortened tests was assessed using linear regression and re-sampling analysis. Validity of reduced-sampling tests was assessed using Pearson's correlation coefficients and Bland-Altman analysis. Results: a) A single-point triacylglycerol concentration (3 or 4h postprandially) did not present significant correlation with postprandial lipemia in the hypetriacylglycerolemic, exercise and energy restriction groups (P>0.05), b) Time-shortened OFTT (4h) was able to predict postprandial lipemia in every group studied (R 2=0.707-0.970, P<0.01), except the hypertriacylglycerolemics (P=0.338), c) Reduced-sampling OFTT (3 or 4 samples) was able to assess postprandial lipemia in every group as well as in the overall sample (r=0.874-0.997, P<0.05). Conclusions: A more convenient, reduced-sampling OFTT may be used alternatively to the conventional OFTT, whereas a time-shortened OFTT may be appropriate only for healthy people.
AB - Background & aims: Postprandial lipemia is assessed using the oral fat tolerance test (OFTT), a six-hour procedure requiring hourly blood sampling. In order to simplify the test, we investigated whether a) a single postprandial triacylglycerol concentration, b) an OFTT lasting less than six hours or c) an OFTT requiring fewer blood samples, may accurately assess postprandial lipemia under various conditions. Methods: Seventy-two subjects underwent a conventional OFTT. Predictability of single-point concentrations and time-shortened tests was assessed using linear regression and re-sampling analysis. Validity of reduced-sampling tests was assessed using Pearson's correlation coefficients and Bland-Altman analysis. Results: a) A single-point triacylglycerol concentration (3 or 4h postprandially) did not present significant correlation with postprandial lipemia in the hypetriacylglycerolemic, exercise and energy restriction groups (P>0.05), b) Time-shortened OFTT (4h) was able to predict postprandial lipemia in every group studied (R 2=0.707-0.970, P<0.01), except the hypertriacylglycerolemics (P=0.338), c) Reduced-sampling OFTT (3 or 4 samples) was able to assess postprandial lipemia in every group as well as in the overall sample (r=0.874-0.997, P<0.05). Conclusions: A more convenient, reduced-sampling OFTT may be used alternatively to the conventional OFTT, whereas a time-shortened OFTT may be appropriate only for healthy people.
KW - Clinical testing
KW - Lipid metabolism
KW - Triglyceride
UR - http://www.scopus.com/inward/record.url?scp=82255163005&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82255163005&partnerID=8YFLogxK
U2 - 10.1016/j.clnu.2011.05.003
DO - 10.1016/j.clnu.2011.05.003
M3 - Article
C2 - 21665336
AN - SCOPUS:82255163005
SN - 0261-5614
VL - 30
SP - 852
EP - 857
JO - Clinical Nutrition
JF - Clinical Nutrition
IS - 6
ER -