TY - JOUR
T1 - Variants of Venezuelan equine encephalitis virus that resist neutralization define a domain of the E2 glycoprotein
AU - Johnson, Barbara J.B.
AU - Brubaker, John R.
AU - Roehrig, John T.
AU - Trent, Dennis W.
PY - 1990/8
Y1 - 1990/8
N2 - Stable neutralization (N) escape variants of Venezuelan equine encephalitis (VEE) virus were selected by anti-E2 glycoprotein monoclonal antibodies (MAbs) that neutralize viral infectivity, block viral hemagglutination, and passively protect mice. The nucleotide sequence of the Et, E2, and E3 genes of four variants revealed a clustering of single mutations in a domain spanning E2-182 to E2-207. The conformation of this short linear sequence affects antigenicity in the N domain because reduction and alkylation of virus disrupted binding of some E2 neutralizing MAbs. Serologic evidence for interaction of E2 epitopes also was obtained. Mutations in the N domain of VEE virus did not alter the kinetics of binding to Vero cells. They did, in some cases, produce attenuation of virulence in mice.
AB - Stable neutralization (N) escape variants of Venezuelan equine encephalitis (VEE) virus were selected by anti-E2 glycoprotein monoclonal antibodies (MAbs) that neutralize viral infectivity, block viral hemagglutination, and passively protect mice. The nucleotide sequence of the Et, E2, and E3 genes of four variants revealed a clustering of single mutations in a domain spanning E2-182 to E2-207. The conformation of this short linear sequence affects antigenicity in the N domain because reduction and alkylation of virus disrupted binding of some E2 neutralizing MAbs. Serologic evidence for interaction of E2 epitopes also was obtained. Mutations in the N domain of VEE virus did not alter the kinetics of binding to Vero cells. They did, in some cases, produce attenuation of virulence in mice.
UR - http://www.scopus.com/inward/record.url?scp=0025348447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025348447&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(90)90533-W
DO - 10.1016/0042-6822(90)90533-W
M3 - Article
C2 - 1695412
AN - SCOPUS:0025348447
SN - 0042-6822
VL - 177
SP - 676
EP - 683
JO - Virology
JF - Virology
IS - 2
ER -