Variation in the biological function of envelope protein epitopes of yellow fever vaccine viruses detected with monoclonal antibodies

T. N. Ledger, B. K. Sil, M. R. Wills, G. Lewis, R. M. Kinney, A. D. Jennings, J. R. Stephenson, A. D.T. Barrett

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Three different virus strains (17D-204, 17DD and the French neurotropic vaccine) have been used as live attenuated yellow fever (YF) vaccines and are manufactured in different centres around the world. The envelope proteins of these vaccine viruses were examined and compared using mouse monoclonal antibodies (MAbs) in haemagglutination inhibition (HAI) and neutralization (N) tests. The epitopes eliciting HAI and/or N were found to vary depending on the virus examined. Such variation was also found between vaccine viruses of the same strain manufactured in different centres. These data were confirmed by the use of mouse polyclonal antisera. On the basis of the MAb results in HAI tests a dendrogram of the similarity coefficients between the viruses was constructed and showed that the viruses could be placed into three major groups. Thus, it is concluded that YF vaccines manufactured in different centres are antigenically distinct as recognized by the mouse immune system.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalBiologicals
Volume20
Issue number2
DOIs
StatePublished - Jun 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Immunology and Microbiology(all)
  • Pharmacology

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