Vasoactive intestinal peptide enhances wound healing and proliferation of human bronchial epithelial cells

Cha Xiang Guan, Min Zhang, Xiao Qun Qin, Yan Ru Cui, Zi Qiang Luo, Hong Bo Bai, Xiang Fang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In the present study, we investigated the effects of vasoactive intestinal peptide (VIP) on wound healing of bronchial epithelium. Wound healing of the mechanical damaged human bronchial epithelial cells (HBEC) was observed in the absence or presence of VIP. Effects of VIP on chemotactic migration, cell proliferation of HBEC were also tested. HBEC chemotaxis was assessed by the blind well chamber technique, the cell cycle was determined by flow cytometry, and cell proliferation was determined by measuring the expression of proliferating cell nuclear antigen Ki67. Effects of VIP on epithelial E-cadherins protein and mRNA were also measured by immunohistochemistry and RT-PCR. The results showed that VIP accelerated the recovery of wound area of HBEC. VIP increased the migration and proliferation of HBEC, and these effects were blocked by a VPAC1 receptor antagonist. VIP also increased the expression of E-cadherin mRNA and protein in HBEC, suggesting that protective effects of VIP on wound healing may be related to its ability to increase the expression of E-cadherin. In conclusion, VIP has protective effects against human bronchial epithelial cell damage, and the beneficial effects of VIP might be mediated, at least in part, by VPAC1, and associated with increased expression of E-cadherin.

Original languageEnglish (US)
Pages (from-to)3107-3114
Number of pages8
JournalPeptides
Volume27
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Vasoactive Intestinal Peptide
Wound Healing
Epithelial Cells
Cadherins
Cell proliferation
Receptors, Vasoactive Intestinal Polypeptide, Type I
Cell Proliferation
Ki-67 Antigen
Messenger RNA
Aptitude
Flow cytometry
Proliferating Cell Nuclear Antigen
Chemotaxis
Cell Cycle
Flow Cytometry
Proteins
Epithelium
Immunohistochemistry
Cells
Recovery

Keywords

  • Human bronchial epithelial cells
  • Proliferation
  • Vasoactive intestinal peptide
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Vasoactive intestinal peptide enhances wound healing and proliferation of human bronchial epithelial cells. / Guan, Cha Xiang; Zhang, Min; Qin, Xiao Qun; Cui, Yan Ru; Luo, Zi Qiang; Bai, Hong Bo; Fang, Xiang.

In: Peptides, Vol. 27, No. 12, 12.2006, p. 3107-3114.

Research output: Contribution to journalArticle

Guan, Cha Xiang ; Zhang, Min ; Qin, Xiao Qun ; Cui, Yan Ru ; Luo, Zi Qiang ; Bai, Hong Bo ; Fang, Xiang. / Vasoactive intestinal peptide enhances wound healing and proliferation of human bronchial epithelial cells. In: Peptides. 2006 ; Vol. 27, No. 12. pp. 3107-3114.
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AU - Bai, Hong Bo

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