Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

Guo Ying Sun, Cha Xiang Guan, Yong Zhou, Yong Ping Liu, Shu Fen Li, Hui Fang Zhou, Chun Yan Tang, Xiang Fang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalRegulatory Peptides
Volume167
Issue number1
DOIs
StatePublished - Feb 25 2011

Keywords

  • Acute lung injury
  • Macrophage
  • TREM-1
  • TREM-2
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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  • Cite this

    Sun, G. Y., Guan, C. X., Zhou, Y., Liu, Y. P., Li, S. F., Zhou, H. F., Tang, C. Y., & Fang, X. (2011). Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury. Regulatory Peptides, 167(1), 56-64. https://doi.org/10.1016/j.regpep.2010.11.008