Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

Guo Ying Sun; Cha Xiang Guan; Yong Zhou; Yong Ping Liu; Shu Fen Li; Hui Fang Zhou; Chun Yan Tang; Xiang Fang

doi:10.1016/j.regpep.2010.11.008

Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

Guo Ying Sun, Cha Xiang Guan, Yong Zhou, Yong Ping Liu, Shu Fen Li, Hui Fang Zhou, Chun Yan Tang, Xiang Fang

Neurology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

Original language	English (US)
Pages (from-to)	56-64
Number of pages	9
Journal	Regulatory Peptides
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/j.regpep.2010.11.008
State	Published - Feb 25 2011

Keywords

Acute lung injury
Macrophage
TREM-1
TREM-2
Vasoactive intestinal peptide

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/j.regpep.2010.11.008

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@article{6af307ffdbf74e74a2d53f00666e54d3,

title = "Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury",

abstract = "Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.",

keywords = "Acute lung injury, Macrophage, TREM-1, TREM-2, Vasoactive intestinal peptide",

author = "Sun, {Guo Ying} and Guan, {Cha Xiang} and Yong Zhou and Liu, {Yong Ping} and Li, {Shu Fen} and Zhou, {Hui Fang} and Tang, {Chun Yan} and Xiang Fang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30870915 ) and Hunan Provincial Innovation Foundation for Postgraduate (NO. CX2010B096 ) and Central South University Open Sharing Foundation for the valuable equipment (No. Zkj2009013 ).We thanks Dr. Gruenert from the University of California, San Francisco, for providing an immortalized human BEC line 16HBE140-.",

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doi = "10.1016/j.regpep.2010.11.008",

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AU - Sun, Guo Ying

AU - Guan, Cha Xiang

AU - Zhou, Yong

AU - Liu, Yong Ping

AU - Li, Shu Fen

AU - Zhou, Hui Fang

AU - Tang, Chun Yan

AU - Fang, Xiang

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30870915 ) and Hunan Provincial Innovation Foundation for Postgraduate (NO. CX2010B096 ) and Central South University Open Sharing Foundation for the valuable equipment (No. Zkj2009013 ).We thanks Dr. Gruenert from the University of California, San Francisco, for providing an immortalized human BEC line 16HBE140-.

PY - 2011/2/25

Y1 - 2011/2/25

N2 - Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

AB - Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

KW - Acute lung injury

KW - Macrophage

KW - TREM-1

KW - TREM-2

KW - Vasoactive intestinal peptide

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Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

Abstract

Keywords

ASJC Scopus subject areas

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