Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

Guo Ying Sun, Cha Xiang Guan, Yong Zhou, Yong Ping Liu, Shu Fen Li, Hui Fang Zhou, Chun Yan Tang, Xiang Fang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalRegulatory Peptides
Volume167
Issue number1
DOIs
StatePublished - Feb 25 2011

Fingerprint

Acute Lung Injury
Vasoactive Intestinal Peptide
Myeloid Cells
Messenger RNA
Lipopolysaccharides
Tissue
Macrophages
Lung
Anti-Inflammatory Agents
Respiratory system
Fibroblasts
Neuropeptides
Animals
Cells

Keywords

  • Acute lung injury
  • Macrophage
  • TREM-1
  • TREM-2
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury. / Sun, Guo Ying; Guan, Cha Xiang; Zhou, Yong; Liu, Yong Ping; Li, Shu Fen; Zhou, Hui Fang; Tang, Chun Yan; Fang, Xiang.

In: Regulatory Peptides, Vol. 167, No. 1, 25.02.2011, p. 56-64.

Research output: Contribution to journalArticle

Sun, GY, Guan, CX, Zhou, Y, Liu, YP, Li, SF, Zhou, HF, Tang, CY & Fang, X 2011, 'Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury', Regulatory Peptides, vol. 167, no. 1, pp. 56-64. https://doi.org/10.1016/j.regpep.2010.11.008
Sun, Guo Ying ; Guan, Cha Xiang ; Zhou, Yong ; Liu, Yong Ping ; Li, Shu Fen ; Zhou, Hui Fang ; Tang, Chun Yan ; Fang, Xiang. / Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury. In: Regulatory Peptides. 2011 ; Vol. 167, No. 1. pp. 56-64.
@article{6af307ffdbf74e74a2d53f00666e54d3,
title = "Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury",
abstract = "Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.",
keywords = "Acute lung injury, Macrophage, TREM-1, TREM-2, Vasoactive intestinal peptide",
author = "Sun, {Guo Ying} and Guan, {Cha Xiang} and Yong Zhou and Liu, {Yong Ping} and Li, {Shu Fen} and Zhou, {Hui Fang} and Tang, {Chun Yan} and Xiang Fang",
year = "2011",
month = "2",
day = "25",
doi = "10.1016/j.regpep.2010.11.008",
language = "English (US)",
volume = "167",
pages = "56--64",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Vasoactive intestinal peptide re-balances TREM-1/TREM-2 ratio in acute lung injury

AU - Sun, Guo Ying

AU - Guan, Cha Xiang

AU - Zhou, Yong

AU - Liu, Yong Ping

AU - Li, Shu Fen

AU - Zhou, Hui Fang

AU - Tang, Chun Yan

AU - Fang, Xiang

PY - 2011/2/25

Y1 - 2011/2/25

N2 - Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

AB - Vasoactive intestinal peptide (VIP) is one of the most plentiful neuropeptides in the lung and it has anti-inflammatory effects in the respiratory system. Triggering receptors expressed on myeloid cells-1 (TREM-1) and triggering receptors expressed on myeloid cells-2 (TREM-2) regulate immune responses to lipopolysaccharide (LPS). In the present study, we tested the expressions of TREM-1 and TREM-2 in various pulmonary cell lines and/or tissue using an animal model of LPS-induced acute lung injury (ALI), and determined the effects of VIP on expression of the TREM-1 and TREM-2 in lung tissues and cells from ALI mice. We found 1) expression of the TREM-1 mRNA from lung tissues of ALI was significantly increased, whereas the expression of TREM-2 mRNA was decreased in these tissues; 2) TREM-1 mRNA was only expressed in macrophages, while TREM-2 mRNA was detected in HBECs, lung fibroblasts, lung adenocarcinoma cells and macrophages; 3) the ratio of TREM-1 mRNA to TREM-2 mRNA was increased in LPS-induced lung tissues and macrophages; 4) VIP inhibited expression of the TREM-1 mRNA in a time- and dose-dependent manner in lung cells from LPS-induced ALI mice; however, it increased expression of the TREM-2 mRNA. As a result of these effects, VIP normalized the ratio of TREM-1 to TREM-2 mRNA in these cells. Our results suggest that VIP might exert its anti-inflammatory effect through a mechanism involved in regulation of expression of the TREM-1 and TREM-2 in LPS-induced ALI.

KW - Acute lung injury

KW - Macrophage

KW - TREM-1

KW - TREM-2

KW - Vasoactive intestinal peptide

UR - http://www.scopus.com/inward/record.url?scp=79951681737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951681737&partnerID=8YFLogxK

U2 - 10.1016/j.regpep.2010.11.008

DO - 10.1016/j.regpep.2010.11.008

M3 - Article

VL - 167

SP - 56

EP - 64

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1

ER -