Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root: Partial purification

M. Hoshino, H. Fujii, K. Iguchi, T. Mochizuchi, C. Yanai-Hara, M. Yokota, G. H. Greeley, N. Yanaihara

    Research output: Contribution to journalArticle

    Abstract

    A previous study has demonstrated the presence of several immunoreactive regulatory peptides in extracts of dried ginseng root powder, and insulinotropic activity in the vasoactive intestinal polypeptide (VIP)-like immunoreactivity-containing fraction of this extract. In the present paper, fresh ginseng root was extracted with dilute acetic acid, and the extracts were subjected to gel filtration. Gel filtration revealed the presence of high and low molecular forms of VIP-like immunoreactivity. Both fractions also contained immunoreactive neuropeptides such as peptide HI (PHI), galanin, neuropeptide Y (NPY) and glucagon-like peptide-1 (GLP-1). Of these peptides, galanin-like immunoreactivity showed the highest concentration. Fractions containing VIP-like immunoreactivity were partially purified by ion exchange chromatography on a Q-Sepharose column to give two VIP-like immunoreactive peaks, which eluted at 0.6 M and 0.7 M NaCl in a gradient of NaCl, respectively.

    Original languageEnglish (US)
    Pages (from-to)19-23
    Number of pages5
    JournalBiomedical Research
    Volume11
    Issue numberSUPPL.
    StatePublished - 1990

    Fingerprint

    Panax
    Vasoactive Intestinal Peptide
    Purification
    Gel Chromatography
    Galanin-Like Peptide
    Peptide PHI
    Gels
    Galanin
    Glucagon-Like Peptide 1
    Neuropeptide Y
    Ion Exchange Chromatography
    Chromatography
    Neuropeptides
    Acetic Acid
    Powders
    Sepharose
    Ion exchange
    Peptides

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Hoshino, M., Fujii, H., Iguchi, K., Mochizuchi, T., Yanai-Hara, C., Yokota, M., ... Yanaihara, N. (1990). Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root: Partial purification. Biomedical Research, 11(SUPPL.), 19-23.

    Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root : Partial purification. / Hoshino, M.; Fujii, H.; Iguchi, K.; Mochizuchi, T.; Yanai-Hara, C.; Yokota, M.; Greeley, G. H.; Yanaihara, N.

    In: Biomedical Research, Vol. 11, No. SUPPL., 1990, p. 19-23.

    Research output: Contribution to journalArticle

    Hoshino, M, Fujii, H, Iguchi, K, Mochizuchi, T, Yanai-Hara, C, Yokota, M, Greeley, GH & Yanaihara, N 1990, 'Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root: Partial purification', Biomedical Research, vol. 11, no. SUPPL., pp. 19-23.
    Hoshino M, Fujii H, Iguchi K, Mochizuchi T, Yanai-Hara C, Yokota M et al. Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root: Partial purification. Biomedical Research. 1990;11(SUPPL.):19-23.
    Hoshino, M. ; Fujii, H. ; Iguchi, K. ; Mochizuchi, T. ; Yanai-Hara, C. ; Yokota, M. ; Greeley, G. H. ; Yanaihara, N. / Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in ginseng root : Partial purification. In: Biomedical Research. 1990 ; Vol. 11, No. SUPPL. pp. 19-23.
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    AU - Fujii, H.

    AU - Iguchi, K.

    AU - Mochizuchi, T.

    AU - Yanai-Hara, C.

    AU - Yokota, M.

    AU - Greeley, G. H.

    AU - Yanaihara, N.

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