Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways

Lida Q. Fuentes, Carlos E. Reyes, José M. Sarmiento, Carolina I. Villanueva, Carlos D. Figueroa, Javier Navarro, Carlos B. González

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Activation of V1a receptor triggers the expression of growth-related immediate-early genes (IEGs), including c-Fos and Egr-1. We found that pre-treatment of rat vascular smooth muscle A-10 cell line with the EGF receptor inhibitor AG1478 or the over-expression of an EGFR dominant negative mutant (HEBCD533) blocked the vasopressin-induced expression of IEGs, suggesting that activation of these early genes mediated by V1a receptor is via transactivation of the EGF receptor. Importantly, the inhibition of the metalloproteinases, which catalyzed the shedding of the EGF receptor agonist HB-EGF, selectively blocked the vasopressin-induced expression c-Fos. On the other hand, the inhibition of c-Src selectively blocked the vasopressin-induced expression of Egr-1. Interestingly, in contrast to the expression of c-Fos, the expression of Egr-1 was mediated via the Ras/MEK/MAPK-dependent signalling pathway. Vasopressin-triggered expression of both genes required the release of intracellular calcium, activation of PKC and β-arrestin 2. These findings demonstrated that vasopressin up-regulated the expression of c-Fos and Erg-1 via transactivation of two distinct EGF receptor-dependent signalling pathways.

Original languageEnglish (US)
Pages (from-to)1642-1650
Number of pages9
JournalCellular Signalling
Volume20
Issue number9
DOIs
StatePublished - Sep 2008

Fingerprint

Immediate-Early Genes
Vasopressins
Epidermal Growth Factor Receptor
Transcriptional Activation
Up-Regulation
Growth
antineoplaston A10
Mitogen-Activated Protein Kinase Kinases
Metalloproteases
Vascular Smooth Muscle
Calcium
Gene Expression
Cell Line
Genes

Keywords

  • EGFR transactivation
  • Immediate early genes
  • Metalloproteinases
  • Mitogen-activated kinases
  • PKC
  • Vasopressin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways. / Fuentes, Lida Q.; Reyes, Carlos E.; Sarmiento, José M.; Villanueva, Carolina I.; Figueroa, Carlos D.; Navarro, Javier; González, Carlos B.

In: Cellular Signalling, Vol. 20, No. 9, 09.2008, p. 1642-1650.

Research output: Contribution to journalArticle

Fuentes, Lida Q. ; Reyes, Carlos E. ; Sarmiento, José M. ; Villanueva, Carolina I. ; Figueroa, Carlos D. ; Navarro, Javier ; González, Carlos B. / Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways. In: Cellular Signalling. 2008 ; Vol. 20, No. 9. pp. 1642-1650.
@article{a040efd2c4184e5ab38d06b0b4567265,
title = "Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways",
abstract = "Activation of V1a receptor triggers the expression of growth-related immediate-early genes (IEGs), including c-Fos and Egr-1. We found that pre-treatment of rat vascular smooth muscle A-10 cell line with the EGF receptor inhibitor AG1478 or the over-expression of an EGFR dominant negative mutant (HEBCD533) blocked the vasopressin-induced expression of IEGs, suggesting that activation of these early genes mediated by V1a receptor is via transactivation of the EGF receptor. Importantly, the inhibition of the metalloproteinases, which catalyzed the shedding of the EGF receptor agonist HB-EGF, selectively blocked the vasopressin-induced expression c-Fos. On the other hand, the inhibition of c-Src selectively blocked the vasopressin-induced expression of Egr-1. Interestingly, in contrast to the expression of c-Fos, the expression of Egr-1 was mediated via the Ras/MEK/MAPK-dependent signalling pathway. Vasopressin-triggered expression of both genes required the release of intracellular calcium, activation of PKC and β-arrestin 2. These findings demonstrated that vasopressin up-regulated the expression of c-Fos and Erg-1 via transactivation of two distinct EGF receptor-dependent signalling pathways.",
keywords = "EGFR transactivation, Immediate early genes, Metalloproteinases, Mitogen-activated kinases, PKC, Vasopressin",
author = "Fuentes, {Lida Q.} and Reyes, {Carlos E.} and Sarmiento, {Jos{\'e} M.} and Villanueva, {Carolina I.} and Figueroa, {Carlos D.} and Javier Navarro and Gonz{\'a}lez, {Carlos B.}",
year = "2008",
month = "9",
doi = "10.1016/j.cellsig.2008.05.009",
language = "English (US)",
volume = "20",
pages = "1642--1650",
journal = "Cellular Signalling",
issn = "0898-6568",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Vasopressin up-regulates the expression of growth-related immediate-early genes via two distinct EGF receptor transactivation pathways

AU - Fuentes, Lida Q.

AU - Reyes, Carlos E.

AU - Sarmiento, José M.

AU - Villanueva, Carolina I.

AU - Figueroa, Carlos D.

AU - Navarro, Javier

AU - González, Carlos B.

PY - 2008/9

Y1 - 2008/9

N2 - Activation of V1a receptor triggers the expression of growth-related immediate-early genes (IEGs), including c-Fos and Egr-1. We found that pre-treatment of rat vascular smooth muscle A-10 cell line with the EGF receptor inhibitor AG1478 or the over-expression of an EGFR dominant negative mutant (HEBCD533) blocked the vasopressin-induced expression of IEGs, suggesting that activation of these early genes mediated by V1a receptor is via transactivation of the EGF receptor. Importantly, the inhibition of the metalloproteinases, which catalyzed the shedding of the EGF receptor agonist HB-EGF, selectively blocked the vasopressin-induced expression c-Fos. On the other hand, the inhibition of c-Src selectively blocked the vasopressin-induced expression of Egr-1. Interestingly, in contrast to the expression of c-Fos, the expression of Egr-1 was mediated via the Ras/MEK/MAPK-dependent signalling pathway. Vasopressin-triggered expression of both genes required the release of intracellular calcium, activation of PKC and β-arrestin 2. These findings demonstrated that vasopressin up-regulated the expression of c-Fos and Erg-1 via transactivation of two distinct EGF receptor-dependent signalling pathways.

AB - Activation of V1a receptor triggers the expression of growth-related immediate-early genes (IEGs), including c-Fos and Egr-1. We found that pre-treatment of rat vascular smooth muscle A-10 cell line with the EGF receptor inhibitor AG1478 or the over-expression of an EGFR dominant negative mutant (HEBCD533) blocked the vasopressin-induced expression of IEGs, suggesting that activation of these early genes mediated by V1a receptor is via transactivation of the EGF receptor. Importantly, the inhibition of the metalloproteinases, which catalyzed the shedding of the EGF receptor agonist HB-EGF, selectively blocked the vasopressin-induced expression c-Fos. On the other hand, the inhibition of c-Src selectively blocked the vasopressin-induced expression of Egr-1. Interestingly, in contrast to the expression of c-Fos, the expression of Egr-1 was mediated via the Ras/MEK/MAPK-dependent signalling pathway. Vasopressin-triggered expression of both genes required the release of intracellular calcium, activation of PKC and β-arrestin 2. These findings demonstrated that vasopressin up-regulated the expression of c-Fos and Erg-1 via transactivation of two distinct EGF receptor-dependent signalling pathways.

KW - EGFR transactivation

KW - Immediate early genes

KW - Metalloproteinases

KW - Mitogen-activated kinases

KW - PKC

KW - Vasopressin

UR - http://www.scopus.com/inward/record.url?scp=46849101053&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46849101053&partnerID=8YFLogxK

U2 - 10.1016/j.cellsig.2008.05.009

DO - 10.1016/j.cellsig.2008.05.009

M3 - Article

C2 - 18571897

AN - SCOPUS:46849101053

VL - 20

SP - 1642

EP - 1650

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 9

ER -