VEGFR-2 expression in carcinoid cancer cells and its role in tumor growth and metastasis

Scott R. Silva, Kanika Bowen-Jallow, Piotr G. Rychahou, Lindsey N. Jackson, Heidi L. Weiss, Eun Y. Lee, Courtney Townsend, B. Mark Evers

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Carcinoid tumors are slow growing and highly vascular neuroendocrine neoplasms that are increasing in incidence. Previously, we showed that carcinoid tumors express vascular endothelial growth factor receptor 2 (VEGFR-2) in the epithelial compartment of carcinoid tumor sections; yet, its role is not completely understood. The purpose of our study was to: (i) assess the expression of VEGFR-2 in the novel human carcinoid cell line BON, (ii) to determine the role of PI3K/Akt signaling on VEGFR-2 expression and (iii) to assess the effect of VEGFR-2 on BON cell invasion, migration and proliferation. We found that, although VEGFR-2 is expressed in BON cells, reduction in VEGFR-2 expression actually enhanced proliferation, invasion, and migration of the BON cell line. Also, expression of VEGFR-2 was inversely related to PI3K signaling. Carcinoid liver metastases in mice demonstrated decreased VEGFR-2 expression. Furthermore, the expression of a truncated, soluble form of VEGFR-2 (sVEGFR-2), a protein demonstrated to inhibit cell growth, was detected in BON cells. The presence of VEGFR-2 in the epithelial component of carcinoid tumors and in the BON cell line suggests an alternate role for VEGFR-2, in addition to its well-defined role in angiogenesis. The expression of sVEGFR-2 may explain the inverse relationship between VEGFR-2 expression and PI3K/Akt signaling and the inhibitory effect VEGFR-2 has on BON cell proliferation, migration and invasion.

Original languageEnglish (US)
Pages (from-to)1045-1056
Number of pages12
JournalInternational Journal of Cancer
Volume128
Issue number5
DOIs
StatePublished - Mar 1 2011

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Vascular Endothelial Growth Factor Receptor-2
Carcinoid Tumor
Neoplasm Metastasis
Growth
Neoplasms
Phosphatidylinositol 3-Kinases
Cell Line
Cell Movement
Vascular Neoplasms
Cell Proliferation

Keywords

  • angiogenic factors and receptors
  • cell motility and migration
  • tumor markers and detection of metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Silva, S. R., Bowen-Jallow, K., Rychahou, P. G., Jackson, L. N., Weiss, H. L., Lee, E. Y., ... Evers, B. M. (2011). VEGFR-2 expression in carcinoid cancer cells and its role in tumor growth and metastasis. International Journal of Cancer, 128(5), 1045-1056. https://doi.org/10.1002/ijc.25441

VEGFR-2 expression in carcinoid cancer cells and its role in tumor growth and metastasis. / Silva, Scott R.; Bowen-Jallow, Kanika; Rychahou, Piotr G.; Jackson, Lindsey N.; Weiss, Heidi L.; Lee, Eun Y.; Townsend, Courtney; Evers, B. Mark.

In: International Journal of Cancer, Vol. 128, No. 5, 01.03.2011, p. 1045-1056.

Research output: Contribution to journalArticle

Silva, Scott R. ; Bowen-Jallow, Kanika ; Rychahou, Piotr G. ; Jackson, Lindsey N. ; Weiss, Heidi L. ; Lee, Eun Y. ; Townsend, Courtney ; Evers, B. Mark. / VEGFR-2 expression in carcinoid cancer cells and its role in tumor growth and metastasis. In: International Journal of Cancer. 2011 ; Vol. 128, No. 5. pp. 1045-1056.
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