Vertebrate attenuated West Nile virus mutants have differing effects on vector competence in Culex tarsalis mosquitoes

Greta A. Van Slyke, Yongqing Jia, Melissa C. Whiteman, Jason A. Wicker, Alan Barrett, Laura D. Kramer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Previous mutational analyses of naturally occurring West Nile virus (WNV) strains and engineered mutant WNV strains have identified locations in the viral genome that can have profound phenotypic effect on viral infectivity, temperature sensitivity and neuroinvasiveness. We chose six mutant WNV strains to evaluate for vector competence in the natural WNV vector Culex tarsalis, two of which contain multiple ablations of glycosylation sites in the envelope and NS1 proteins; three of which contain mutations in the NS4B protein and an attenuated natural bird isolate (Bird 1153) harbouring an NS4B mutation. Despite vertebrate attenuation, all NS4B mutant viruses displayed enhanced vector competence by Cx. tarsalis. Non-glycosylated mutant viruses displayed decreased vector competence in Cx. tarsalis mosquitoes, particularly when all three NS1 glycosylation sites were abolished. These results indicate the importance of both the NS4B protein and NS1 glycosylation in the transmission of WNV by a significant mosquito vector.

Original languageEnglish (US)
Pages (from-to)1069-1072
Number of pages4
JournalJournal of General Virology
Volume94
Issue numberPART 5
DOIs
StatePublished - May 2013

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West Nile virus
Culex
Culicidae
Mental Competency
Vertebrates
Glycosylation
Birds
Viruses
Mutation
Viral Genome
Proteins
Temperature

ASJC Scopus subject areas

  • Virology

Cite this

Vertebrate attenuated West Nile virus mutants have differing effects on vector competence in Culex tarsalis mosquitoes. / Van Slyke, Greta A.; Jia, Yongqing; Whiteman, Melissa C.; Wicker, Jason A.; Barrett, Alan; Kramer, Laura D.

In: Journal of General Virology, Vol. 94, No. PART 5, 05.2013, p. 1069-1072.

Research output: Contribution to journalArticle

Van Slyke, Greta A. ; Jia, Yongqing ; Whiteman, Melissa C. ; Wicker, Jason A. ; Barrett, Alan ; Kramer, Laura D. / Vertebrate attenuated West Nile virus mutants have differing effects on vector competence in Culex tarsalis mosquitoes. In: Journal of General Virology. 2013 ; Vol. 94, No. PART 5. pp. 1069-1072.
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