Vibrio harveyi NADPH:FMN oxidoreductase: Preparation and characterization of the apoenzyme and monomer-dimer equilibrium

Mengyao Liu, Benfang Lie, Qizhu Ding, J. Ching Lee, Shiao Chun Tu

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A rapid chromatography method was developed for the preparation of apoenzyme of Vibrio harveyi NADPH:FMN oxidoreductase with ≤80% yields. The apoenzyme bound one FMN per enzyme monomer with a dissociation constant of 0.2 μM at 23°C. The reconstituted holoenzyme was catalytically as active as the native enzyme. FMN binding resulted in 87 and 92% of quenching of protein and flavin fluorescence, respectively, indicating a conformational difference between the apoprotein and the holoenzyme. Neither riboflavin nor FAD showed any appreciable binding to the cofactor site of the apoenzyme but both flavins were active substrates for the FMN-containing holoenzyme, 2ThioFMN bound to the cofactor site of the apoenzyme with an affinity similar to that for FMN binding. The holoenzyme reconstituted with 2-thioFMN showed a 509-nm absorption peak, which represents a 19-nm red shift from the corresponding peak of the free flavin, and was catalytically active in using either FMN or 2thioFMN as a substrate. The holoenzyme showed a concentration dependence in molecular sieve chromatography corresponding to higher apparent molecular weights at higher concentrations. Both the holoenzyme and the apoenzyme was shown at 4°C by equilibrium ultracentrifugation to undergo dimerizationwith dissociation constants of 1.8 and 3.3 μM, respectively.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume337
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • NADPH:FMN oxidoreductase
  • apoenzyme
  • dimerization
  • flavin reductase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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