Viral capsid mobility

A dynamic conduit for inactivation

Klas Broo, Jing Wei, Dawn Marshall, Fred Brown, Thomas Smith, John E. Johnson, Anette Schneemann, Gary Siuzdak

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Mass spectrometry and fluorescent probes have provided direct evidence that alkylating agents permeate the protein capsid of naked viruses and chemically inactivate the nucleic acid. N-acetylaziridine and a fluorescent alkylating agent, dansyl sulfonate aziridine, inactivated three different viruses, flock house virus, human rhinovirus-14, and foot and mouth disease virus. Mass spectral studies as well as fluorescent probes showed that alkylation of the genome was the mechanism of inactivation. Because particle integrity was not affected by selective alkylation (as shown by electron microscopy and sucrose gradient experiments), it was reasoned that the dynamic nature of the viral capsid acts as a conduit to the interior of the particle. Potential applications include fluorescent labeling for imaging viral genomes in living cells, the sterilization of blood products, vaccine development, and viral inactivation in vivo.

Original languageEnglish (US)
Pages (from-to)2274-2277
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number5
DOIs
StatePublished - Feb 27 2001
Externally publishedYes

Fingerprint

Capsid
Fluorescent Dyes
Alkylating Agents
Alkylation
Viruses
Virus Inactivation
Foot-and-Mouth Disease Virus
Rhinovirus
Viral Genome
Capsid Proteins
Nucleic Acids
Sucrose
Blood Cells
Mass Spectrometry
Electron Microscopy
Vaccines
Genome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Viral capsid mobility : A dynamic conduit for inactivation. / Broo, Klas; Wei, Jing; Marshall, Dawn; Brown, Fred; Smith, Thomas; Johnson, John E.; Schneemann, Anette; Siuzdak, Gary.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 5, 27.02.2001, p. 2274-2277.

Research output: Contribution to journalArticle

Broo, K, Wei, J, Marshall, D, Brown, F, Smith, T, Johnson, JE, Schneemann, A & Siuzdak, G 2001, 'Viral capsid mobility: A dynamic conduit for inactivation', Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 5, pp. 2274-2277. https://doi.org/10.1073/pnas.051598298
Broo, Klas ; Wei, Jing ; Marshall, Dawn ; Brown, Fred ; Smith, Thomas ; Johnson, John E. ; Schneemann, Anette ; Siuzdak, Gary. / Viral capsid mobility : A dynamic conduit for inactivation. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 5. pp. 2274-2277.
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