Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly

Muhammad Shah Nawaz-ul-Rehman, K. Reddisiva Prasanth, Kai Xu, Zsuzsanna Sasvari, Nikolay Kovalev, Isabel Fernández de Castro Martín, Daniel Barajas, Cristina Risco, Peter D. Nagy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

RNA viruses exploit host cells by co-opting host factors and lipids and escaping host antiviral responses. Previous genome-wide screens with Tomato bushy stunt virus (TBSV) in the model host yeast have identified 18 cellular genes that are part of the actin network. In this paper, we show that the p33 viral replication factor interacts with the cellular cofilin (Cof1p), which is an actin depolymerization factor. Using temperature-sensitive (ts) Cof1p or actin (Act1p) mutants at a semi-permissive temperature, we find an increased level of TBSV RNA accumulation in yeast cells and elevated in vitro activity of the tombusvirus replicase. We show that the large p33 containing replication organelle-like structures are located in the close vicinity of actin patches in yeast cells or around actin cable hubs in infected plant cells. Therefore, the actin filaments could be involved in VRC assembly and the formation of large viral replication compartments containing many individual VRCs. Moreover, we show that the actin network affects the recruitment of viral and cellular components, including oxysterol binding proteins and VAP proteins to form membrane contact sites for efficient transfer of sterols to the sites of replication. Altogether, the emerging picture is that TBSV, via direct interaction between the p33 replication protein and Cof1p, controls cofilin activities to obstruct the dynamic actin network that leads to efficient subversion of cellular factors for pro-viral functions. In summary, the discovery that TBSV interacts with cellular cofilin and blocks the severing of existing filaments and the formation of new actin filaments in infected cells opens a new window to unravel the way by which viruses could subvert/co-opt cellular proteins and lipids. By regulating the functions of cofilin and the actin network, which are central nodes in cellular pathways, viruses could gain supremacy in subversion of cellular factors for pro-viral functions.

Original languageEnglish (US)
Article numbere1005440
JournalPLoS Pathogens
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Actin Depolymerizing Factors
Virus Assembly
Viral Proteins
Tombusvirus
Actins
Yeasts
Actin Cytoskeleton
Viruses
Lipids
Viral Structures
Proteins
Temperature
RNA Viruses
Plant Cells
Sterols
Organelles
Antiviral Agents
Genome
RNA
Membranes

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Nawaz-ul-Rehman, M. S., Prasanth, K. R., Xu, K., Sasvari, Z., Kovalev, N., de Castro Martín, I. F., ... Nagy, P. D. (2016). Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly. PLoS Pathogens, 12(2), [e1005440]. https://doi.org/10.1371/journal.ppat.1005440

Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly. / Nawaz-ul-Rehman, Muhammad Shah; Prasanth, K. Reddisiva; Xu, Kai; Sasvari, Zsuzsanna; Kovalev, Nikolay; de Castro Martín, Isabel Fernández; Barajas, Daniel; Risco, Cristina; Nagy, Peter D.

In: PLoS Pathogens, Vol. 12, No. 2, e1005440, 01.02.2016.

Research output: Contribution to journalArticle

Nawaz-ul-Rehman, MS, Prasanth, KR, Xu, K, Sasvari, Z, Kovalev, N, de Castro Martín, IF, Barajas, D, Risco, C & Nagy, PD 2016, 'Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly', PLoS Pathogens, vol. 12, no. 2, e1005440. https://doi.org/10.1371/journal.ppat.1005440
Nawaz-ul-Rehman, Muhammad Shah ; Prasanth, K. Reddisiva ; Xu, Kai ; Sasvari, Zsuzsanna ; Kovalev, Nikolay ; de Castro Martín, Isabel Fernández ; Barajas, Daniel ; Risco, Cristina ; Nagy, Peter D. / Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly. In: PLoS Pathogens. 2016 ; Vol. 12, No. 2.
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