Viremia and antibody response to La Crosse virus in sentinel gray squirrels (sciuris carolinensis) and chipmunks (Tamias striatus)

Thomas Ksiazek, T. M. Yuill

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Six isolates of La Crosse (LAC) virus were obtained from sentinel gray squirrels (Sciuris carolinensis) and four from sentinel chipmunks (Tamias striatus) in an endemic area. Viremia titers were measured by plaquing on Vero cells. Antibody responses of the animals were measured by a microneutralization test employing four California group viruses: LAC, snowshoe hare (SSH), trivittatus, and Jamestown Canyon. In both species LAC antibody titers peaked at approximately 21 days and were still detectable in all animals at 256 days post viremia. In chipmunks, homologous LAC virus antibody levels were consistently higher than heterologous antibody responses throughout the period recorded. However, in squirrels, homologous LAC virus and heterologous SSH virus antibody responses were initially comparable. This heterologous SSH titer rapidly declined while LAC antibody levels remained relatively high. Data indicate that antibody response persists from one summer season to the next. Viremia titers in both species indicate that these two species are capable of infecting Aedes triseriatus, the principal vector of LAC virus. This is the first reported field isolation of LAC virus from the squirrel.

Original languageEnglish
Pages (from-to)815-821
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume26
Issue number4
StatePublished - 1977
Externally publishedYes

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La Crosse virus
Sciuridae
Viremia
Antibody Formation
Hares
Antibodies
California Encephalitis Viruses
Heterophile Antibodies
Vero Cells
Aedes
cyhalothrin
Viruses

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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abstract = "Six isolates of La Crosse (LAC) virus were obtained from sentinel gray squirrels (Sciuris carolinensis) and four from sentinel chipmunks (Tamias striatus) in an endemic area. Viremia titers were measured by plaquing on Vero cells. Antibody responses of the animals were measured by a microneutralization test employing four California group viruses: LAC, snowshoe hare (SSH), trivittatus, and Jamestown Canyon. In both species LAC antibody titers peaked at approximately 21 days and were still detectable in all animals at 256 days post viremia. In chipmunks, homologous LAC virus antibody levels were consistently higher than heterologous antibody responses throughout the period recorded. However, in squirrels, homologous LAC virus and heterologous SSH virus antibody responses were initially comparable. This heterologous SSH titer rapidly declined while LAC antibody levels remained relatively high. Data indicate that antibody response persists from one summer season to the next. Viremia titers in both species indicate that these two species are capable of infecting Aedes triseriatus, the principal vector of LAC virus. This is the first reported field isolation of LAC virus from the squirrel.",
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PY - 1977

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N2 - Six isolates of La Crosse (LAC) virus were obtained from sentinel gray squirrels (Sciuris carolinensis) and four from sentinel chipmunks (Tamias striatus) in an endemic area. Viremia titers were measured by plaquing on Vero cells. Antibody responses of the animals were measured by a microneutralization test employing four California group viruses: LAC, snowshoe hare (SSH), trivittatus, and Jamestown Canyon. In both species LAC antibody titers peaked at approximately 21 days and were still detectable in all animals at 256 days post viremia. In chipmunks, homologous LAC virus antibody levels were consistently higher than heterologous antibody responses throughout the period recorded. However, in squirrels, homologous LAC virus and heterologous SSH virus antibody responses were initially comparable. This heterologous SSH titer rapidly declined while LAC antibody levels remained relatively high. Data indicate that antibody response persists from one summer season to the next. Viremia titers in both species indicate that these two species are capable of infecting Aedes triseriatus, the principal vector of LAC virus. This is the first reported field isolation of LAC virus from the squirrel.

AB - Six isolates of La Crosse (LAC) virus were obtained from sentinel gray squirrels (Sciuris carolinensis) and four from sentinel chipmunks (Tamias striatus) in an endemic area. Viremia titers were measured by plaquing on Vero cells. Antibody responses of the animals were measured by a microneutralization test employing four California group viruses: LAC, snowshoe hare (SSH), trivittatus, and Jamestown Canyon. In both species LAC antibody titers peaked at approximately 21 days and were still detectable in all animals at 256 days post viremia. In chipmunks, homologous LAC virus antibody levels were consistently higher than heterologous antibody responses throughout the period recorded. However, in squirrels, homologous LAC virus and heterologous SSH virus antibody responses were initially comparable. This heterologous SSH titer rapidly declined while LAC antibody levels remained relatively high. Data indicate that antibody response persists from one summer season to the next. Viremia titers in both species indicate that these two species are capable of infecting Aedes triseriatus, the principal vector of LAC virus. This is the first reported field isolation of LAC virus from the squirrel.

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