Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent

Rafael Kroon Campos, Ketyllen Reis Andrade, Paulo Cesar Peregrino Ferreira, Cláudio Antônio Bonjardim, Bernard La Scola, Erna Geessien Kroon, Jônatas Santos Abrahão

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Acanthamoeba polyphaga mimivirus (APMV), the largest known virus, has been studied as a putative pneumonia agent, especially in hospital environments. Despite the repercussions of the discovery of APMV, there has been no study related to the control of APMV and the susceptibility of this virus to disinfectants. Objectives: This work investigated the virucidal activity against mimivirus of chemical biocides commonly used in clinical practice for the disinfection of hospital equipment and rooms. Study design: APMV was dried on sterilized steel coupons, exposed to different concentrations of alcohols (ethanol, 1-propanol and 2-propanol) and commercial disinfectants (active chlorine, glutaraldehyde and benzalkonium chloride) and titrated in amoebas using the TCID50 value. The stability of APMV on an inanimate surface was also tested in the presence and absence of organic matter for 30 days. Results: APMV showed a high level of resistance to chemical biocides, especially alcohols. Only active chlorine and glutaraldehyde were able to decrease the APMV titers to undetectable levels. Dried APMV showed long-lasting stability on an inanimate surface (30 days), even in the absence of organic matter. Conclusions: The data presented herein may help health and laboratory workers plan the best strategy to control this putative pneumonia agent from surfaces and devices.

Original languageEnglish (US)
Pages (from-to)323-328
Number of pages6
JournalJournal of Clinical Virology
Volume55
Issue number4
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Mimiviridae
Disinfectants
Pneumonia
Chlorine
Glutaral
Alcohols
Hospital Equipment and Supplies
Benzalkonium Compounds
Viruses
1-Propanol
Amoeba
2-Propanol
Steel
Disinfection
Ethanol

Keywords

  • Acanthamoeba polyphaga mimivirus
  • Disinfectants
  • Giant viruses
  • Nosocomial infection
  • Pneumonia

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Kroon Campos, R., Andrade, K. R., Ferreira, P. C. P., Bonjardim, C. A., La Scola, B., Kroon, E. G., & Abrahão, J. S. (2012). Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent. Journal of Clinical Virology, 55(4), 323-328. https://doi.org/10.1016/j.jcv.2012.08.009

Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent. / Kroon Campos, Rafael; Andrade, Ketyllen Reis; Ferreira, Paulo Cesar Peregrino; Bonjardim, Cláudio Antônio; La Scola, Bernard; Kroon, Erna Geessien; Abrahão, Jônatas Santos.

In: Journal of Clinical Virology, Vol. 55, No. 4, 01.12.2012, p. 323-328.

Research output: Contribution to journalArticle

Kroon Campos, R, Andrade, KR, Ferreira, PCP, Bonjardim, CA, La Scola, B, Kroon, EG & Abrahão, JS 2012, 'Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent', Journal of Clinical Virology, vol. 55, no. 4, pp. 323-328. https://doi.org/10.1016/j.jcv.2012.08.009
Kroon Campos, Rafael ; Andrade, Ketyllen Reis ; Ferreira, Paulo Cesar Peregrino ; Bonjardim, Cláudio Antônio ; La Scola, Bernard ; Kroon, Erna Geessien ; Abrahão, Jônatas Santos. / Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent. In: Journal of Clinical Virology. 2012 ; Vol. 55, No. 4. pp. 323-328.
@article{109cf886972443f1868cddd3a4bf6fca,
title = "Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent",
abstract = "Background: Acanthamoeba polyphaga mimivirus (APMV), the largest known virus, has been studied as a putative pneumonia agent, especially in hospital environments. Despite the repercussions of the discovery of APMV, there has been no study related to the control of APMV and the susceptibility of this virus to disinfectants. Objectives: This work investigated the virucidal activity against mimivirus of chemical biocides commonly used in clinical practice for the disinfection of hospital equipment and rooms. Study design: APMV was dried on sterilized steel coupons, exposed to different concentrations of alcohols (ethanol, 1-propanol and 2-propanol) and commercial disinfectants (active chlorine, glutaraldehyde and benzalkonium chloride) and titrated in amoebas using the TCID50 value. The stability of APMV on an inanimate surface was also tested in the presence and absence of organic matter for 30 days. Results: APMV showed a high level of resistance to chemical biocides, especially alcohols. Only active chlorine and glutaraldehyde were able to decrease the APMV titers to undetectable levels. Dried APMV showed long-lasting stability on an inanimate surface (30 days), even in the absence of organic matter. Conclusions: The data presented herein may help health and laboratory workers plan the best strategy to control this putative pneumonia agent from surfaces and devices.",
keywords = "Acanthamoeba polyphaga mimivirus, Disinfectants, Giant viruses, Nosocomial infection, Pneumonia",
author = "{Kroon Campos}, Rafael and Andrade, {Ketyllen Reis} and Ferreira, {Paulo Cesar Peregrino} and Bonjardim, {Cl{\'a}udio Ant{\^o}nio} and {La Scola}, Bernard and Kroon, {Erna Geessien} and Abrah{\~a}o, {J{\^o}natas Santos}",
year = "2012",
month = "12",
day = "1",
doi = "10.1016/j.jcv.2012.08.009",
language = "English (US)",
volume = "55",
pages = "323--328",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Virucidal activity of chemical biocides against mimivirus, a putative pneumonia agent

AU - Kroon Campos, Rafael

AU - Andrade, Ketyllen Reis

AU - Ferreira, Paulo Cesar Peregrino

AU - Bonjardim, Cláudio Antônio

AU - La Scola, Bernard

AU - Kroon, Erna Geessien

AU - Abrahão, Jônatas Santos

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Background: Acanthamoeba polyphaga mimivirus (APMV), the largest known virus, has been studied as a putative pneumonia agent, especially in hospital environments. Despite the repercussions of the discovery of APMV, there has been no study related to the control of APMV and the susceptibility of this virus to disinfectants. Objectives: This work investigated the virucidal activity against mimivirus of chemical biocides commonly used in clinical practice for the disinfection of hospital equipment and rooms. Study design: APMV was dried on sterilized steel coupons, exposed to different concentrations of alcohols (ethanol, 1-propanol and 2-propanol) and commercial disinfectants (active chlorine, glutaraldehyde and benzalkonium chloride) and titrated in amoebas using the TCID50 value. The stability of APMV on an inanimate surface was also tested in the presence and absence of organic matter for 30 days. Results: APMV showed a high level of resistance to chemical biocides, especially alcohols. Only active chlorine and glutaraldehyde were able to decrease the APMV titers to undetectable levels. Dried APMV showed long-lasting stability on an inanimate surface (30 days), even in the absence of organic matter. Conclusions: The data presented herein may help health and laboratory workers plan the best strategy to control this putative pneumonia agent from surfaces and devices.

AB - Background: Acanthamoeba polyphaga mimivirus (APMV), the largest known virus, has been studied as a putative pneumonia agent, especially in hospital environments. Despite the repercussions of the discovery of APMV, there has been no study related to the control of APMV and the susceptibility of this virus to disinfectants. Objectives: This work investigated the virucidal activity against mimivirus of chemical biocides commonly used in clinical practice for the disinfection of hospital equipment and rooms. Study design: APMV was dried on sterilized steel coupons, exposed to different concentrations of alcohols (ethanol, 1-propanol and 2-propanol) and commercial disinfectants (active chlorine, glutaraldehyde and benzalkonium chloride) and titrated in amoebas using the TCID50 value. The stability of APMV on an inanimate surface was also tested in the presence and absence of organic matter for 30 days. Results: APMV showed a high level of resistance to chemical biocides, especially alcohols. Only active chlorine and glutaraldehyde were able to decrease the APMV titers to undetectable levels. Dried APMV showed long-lasting stability on an inanimate surface (30 days), even in the absence of organic matter. Conclusions: The data presented herein may help health and laboratory workers plan the best strategy to control this putative pneumonia agent from surfaces and devices.

KW - Acanthamoeba polyphaga mimivirus

KW - Disinfectants

KW - Giant viruses

KW - Nosocomial infection

KW - Pneumonia

UR - http://www.scopus.com/inward/record.url?scp=84867881014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867881014&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2012.08.009

DO - 10.1016/j.jcv.2012.08.009

M3 - Article

VL - 55

SP - 323

EP - 328

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 4

ER -