Virulence factors profiles and ESBL production in Escherichia coli causing bacteremia in Peruvian children

Noemí Palma, Cláudia Gomes, Maribel Riveros, Wilfredo García, Sandra Martínez-Puchol, Lidia Ruiz-Roldán, Judit Mateu, Coralith García, Jan Jacobs, Theresa J. Ochoa, Joaquim Ruiz

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The presence of 25 virulence genes (VGs), genetic phylogroups, quinolone-resistance and Extended Spectrum β-lactamase (ESBL)-production was assessed in 65 Escherichia coli isolates from blood cultures in children <5 years in Peru. The most frequent VGs were fimA (89.2%), iutA (83.1%), agn43 (72.3%), iucA (67.7%), and fyuA (49.2%). The isolates belonged to D (47.7%), A (26.1%), B1 (21.5%), and B2 (4.6%) phylogroups. D + B2 isolates presented a high number of fimA, hly, papC, sat, and fyuA genes. Quinolone-susceptible (22 isolates – 33.8%) and ESBL-negative (31 isolates – 47.7%) isolates carried more VGs that their respective counterparts (5.7 vs. 4.7 and 5.3 vs. 4.4 respectively); the frequency of the fyuA, aat, aap, and hly genes significantly differed between quinolone-resistant and quinolone-susceptible isolates. Neonatal sepsis isolates tended to be more quinolone-resistant (P = 0.0697) and ESBL-producers (P = 0.0776). Early-onset neonatal sepsis isolates possessed a high number of VGs (5.2 VGs), especially in neonates of ≤1 day (5.9 VGs).

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume86
Issue number1
DOIs
StatePublished - Sep 1 2016

Keywords

  • Bacteremia
  • ESBL
  • Extra-intestinal Escherichia coli
  • Neonatal sepsis
  • Quinolone-resistance
  • Virulence

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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