Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital

Nam Leong Hoe, Peng Chan Kwai, Ali S. Khan, Lynette Oon, Yun Se Thoe Su, Lai Bai Xin, Daniel Yeo, Sin Leo Yee, Brenda Ang, Thomas Ksiazek, Ee Ling Ai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). In a longitudinal cross-sectional study, we determined the prevalence of virus in bodily excretions and time of seroconversion in discharged patients with SARS. Conjunctival, throat, stool, and urine specimens were collected weekly from 64 patients and tested for SARS-CoV RNA by real-time polymerase chain reaction; serum samples were collected weekly and tested for SARS-CoV antibody with indirect enzyme immunoassay and immunofluorescence assay. In total, 126 conjunctival, 124 throat swab, 116 stool, and 124 urine specimens were analyzed. Five patients had positive stool samples, collected in weeks 5-9. Two patients seroconverted in weeks 7 and 8; the others were seropositive at the first serum sample collection. In this study, 5 (7.8%) of 64 patients continued to shed viral RNA in stool samples only, for up to week 8 of illness. Most seroconversions occurred by week 6 of illness.

Original languageEnglish (US)
Pages (from-to)1745-1750
Number of pages6
JournalEmerging Infectious Diseases
Volume10
Issue number10
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Severe Acute Respiratory Syndrome
RNA Viruses
Antibodies
Pharynx
Urine
Coronavirus
Viral RNA
Enzyme Assays
Indirect Fluorescent Antibody Technique
Serum
Immunoenzyme Techniques
Real-Time Polymerase Chain Reaction
Cross-Sectional Studies
RNA
Viruses

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Hoe, N. L., Kwai, P. C., Khan, A. S., Oon, L., Su, Y. S. T., Xin, L. B., ... Ai, E. L. (2004). Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital. Emerging Infectious Diseases, 10(10), 1745-1750.

Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital. / Hoe, Nam Leong; Kwai, Peng Chan; Khan, Ali S.; Oon, Lynette; Su, Yun Se Thoe; Xin, Lai Bai; Yeo, Daniel; Yee, Sin Leo; Ang, Brenda; Ksiazek, Thomas; Ai, Ee Ling.

In: Emerging Infectious Diseases, Vol. 10, No. 10, 10.2004, p. 1745-1750.

Research output: Contribution to journalArticle

Hoe, NL, Kwai, PC, Khan, AS, Oon, L, Su, YST, Xin, LB, Yeo, D, Yee, SL, Ang, B, Ksiazek, T & Ai, EL 2004, 'Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital', Emerging Infectious Diseases, vol. 10, no. 10, pp. 1745-1750.
Hoe NL, Kwai PC, Khan AS, Oon L, Su YST, Xin LB et al. Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital. Emerging Infectious Diseases. 2004 Oct;10(10):1745-1750.
Hoe, Nam Leong ; Kwai, Peng Chan ; Khan, Ali S. ; Oon, Lynette ; Su, Yun Se Thoe ; Xin, Lai Bai ; Yeo, Daniel ; Yee, Sin Leo ; Ang, Brenda ; Ksiazek, Thomas ; Ai, Ee Ling. / Virus-specific RNA and antibody from convalescent-phase SARS patients discharged from hospital. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 10. pp. 1745-1750.
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