Visceral and somatic hypersensitivity in TNBS-induced colitis in rats

Qi Qi Zhou, Donald D. Price, Robert M. Caudle, G. Nicholas Verne

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200-250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2-28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14-28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.

Original languageEnglish (US)
Pages (from-to)429-435
Number of pages7
JournalDigestive Diseases and Sciences
Issue number2
StatePublished - Feb 2008
Externally publishedYes


  • Irritable bowel syndrome
  • Somatic hypersensitivity
  • Trinitrobenzene sulfonic acid induced colitis
  • Visceral hypersensitivity
  • Viscerosomatic convergence

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology


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