Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients

Xiao Yan Zhou, Ming Li, Xia Li, Xin Long, Xiu Li Zuo, Xiao Hua Hou, Yingzi Cong, Yan Qing Li

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients.

METHODS: Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort.

RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls.

CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.

Original languageEnglish (US)
Pages (from-to)5211-5227
Number of pages17
JournalWorld Journal of Gastroenterology
Volume22
Issue number22
DOIs
StatePublished - Jun 14 2016
Externally publishedYes

Fingerprint

Dysbiosis
Irritable Bowel Syndrome
Mothers
Fusobacterium
Hypersensitivity
Clostridium
Microbiota
Abdominal Reflex
16S Ribosomal RNA
Control Groups
Volatile Fatty Acids
Electromyography
rRNA Genes
Biomarkers

Keywords

  • 16S rRNA gene
  • Animal model
  • Irritable bowel syndrome
  • Microbiota
  • Pyrosequencing

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients. / Zhou, Xiao Yan; Li, Ming; Li, Xia; Long, Xin; Zuo, Xiu Li; Hou, Xiao Hua; Cong, Yingzi; Li, Yan Qing.

In: World Journal of Gastroenterology, Vol. 22, No. 22, 14.06.2016, p. 5211-5227.

Research output: Contribution to journalArticle

Zhou, Xiao Yan ; Li, Ming ; Li, Xia ; Long, Xin ; Zuo, Xiu Li ; Hou, Xiao Hua ; Cong, Yingzi ; Li, Yan Qing. / Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients. In: World Journal of Gastroenterology. 2016 ; Vol. 22, No. 22. pp. 5211-5227.
@article{a00c350776a842698ea01d0ff906f6d7,
title = "Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients",
abstract = "AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients.METHODS: Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort.RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls.CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.",
keywords = "16S rRNA gene, Animal model, Irritable bowel syndrome, Microbiota, Pyrosequencing",
author = "Zhou, {Xiao Yan} and Ming Li and Xia Li and Xin Long and Zuo, {Xiu Li} and Hou, {Xiao Hua} and Yingzi Cong and Li, {Yan Qing}",
year = "2016",
month = "6",
day = "14",
doi = "10.3748/wjg.v22.i22.5211",
language = "English (US)",
volume = "22",
pages = "5211--5227",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "22",

}

TY - JOUR

T1 - Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients

AU - Zhou, Xiao Yan

AU - Li, Ming

AU - Li, Xia

AU - Long, Xin

AU - Zuo, Xiu Li

AU - Hou, Xiao Hua

AU - Cong, Yingzi

AU - Li, Yan Qing

PY - 2016/6/14

Y1 - 2016/6/14

N2 - AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients.METHODS: Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort.RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls.CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.

AB - AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients.METHODS: Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort.RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls.CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.

KW - 16S rRNA gene

KW - Animal model

KW - Irritable bowel syndrome

KW - Microbiota

KW - Pyrosequencing

UR - http://www.scopus.com/inward/record.url?scp=85006495035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006495035&partnerID=8YFLogxK

U2 - 10.3748/wjg.v22.i22.5211

DO - 10.3748/wjg.v22.i22.5211

M3 - Article

C2 - 27298564

AN - SCOPUS:85006495035

VL - 22

SP - 5211

EP - 5227

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 22

ER -