Abstract
Background/Aims Abnormal visceral sensitivity and disordered motility are common in patients with diabetes mellitus. The purpose of the present study was to investigate whether visceral sensation and bowel motility were altered in a rat model of type 2 diabetes mellitus accompanied by weight loss. Methods A type 2 diabetic rat model in adulthood was developed by administrating streptozotocin (STZ; 90 mg/kg, i.p.) to neonatal rats. Eight weeks after STZ administration, rats with blood glucose level of 200 mg/dL or higher were selected and used as diabetic group (n = 35) in this study. Abdominal withdrawal reflex and arterial pulse rate were measured to examine visceral nociception induced by colorectal distension (0.1-1.0 mL). The amplitude, frequency, and area under the curve (AUC) of spontaneous phasic contractions of colonic circular muscles were recorded in vitro to examine colonic motility. Results STZ-treated diabetic rats gained significantly less weight for 8 weeks than control (P <0.01). Forty-eight percent of the diabetic rats showed enhanced visceral nociceptive response to colorectal distension. Diabetic rats did not differ from control rats in colorectal compliance. However, the frequency and AUC, not the amplitude, of colonic spontaneous contraction in vitro was significantly decreased in diabetic rats compared to control rats (P <0.01 in frequency and P <0.05 in AUC). Conclusions These results demonstrate visceral hypersensitivity and colonic dysmotility in a rat model of type 2 diabetes mellitus accompanied by weight loss.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 581-588 |
| Number of pages | 8 |
| Journal | Journal of Neurogastroenterology and Motility |
| Volume | 21 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2015 |
| Externally published | Yes |
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Keywords
- Abdominal withdrawal reflex
- Colonic dysmotility
- Colorectal distension
- Diabetes mellitus, type 2
- Visceral hypersensitivity
ASJC Scopus subject areas
- Gastroenterology
- Clinical Neurology
Cite this
Visceral hypersensitivity and altered colonic motility in type 2 diabetic rat. / Sung, Tae Sik; La, Jun-Ho; Kang, Tong Mook; Kim, Tae Wan; Yang, Il Suk.
In: Journal of Neurogastroenterology and Motility, Vol. 21, No. 4, 2015, p. 581-588.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visceral hypersensitivity and altered colonic motility in type 2 diabetic rat
AU - Sung, Tae Sik
AU - La, Jun-Ho
AU - Kang, Tong Mook
AU - Kim, Tae Wan
AU - Yang, Il Suk
PY - 2015
Y1 - 2015
N2 - Background/Aims Abnormal visceral sensitivity and disordered motility are common in patients with diabetes mellitus. The purpose of the present study was to investigate whether visceral sensation and bowel motility were altered in a rat model of type 2 diabetes mellitus accompanied by weight loss. Methods A type 2 diabetic rat model in adulthood was developed by administrating streptozotocin (STZ; 90 mg/kg, i.p.) to neonatal rats. Eight weeks after STZ administration, rats with blood glucose level of 200 mg/dL or higher were selected and used as diabetic group (n = 35) in this study. Abdominal withdrawal reflex and arterial pulse rate were measured to examine visceral nociception induced by colorectal distension (0.1-1.0 mL). The amplitude, frequency, and area under the curve (AUC) of spontaneous phasic contractions of colonic circular muscles were recorded in vitro to examine colonic motility. Results STZ-treated diabetic rats gained significantly less weight for 8 weeks than control (P <0.01). Forty-eight percent of the diabetic rats showed enhanced visceral nociceptive response to colorectal distension. Diabetic rats did not differ from control rats in colorectal compliance. However, the frequency and AUC, not the amplitude, of colonic spontaneous contraction in vitro was significantly decreased in diabetic rats compared to control rats (P <0.01 in frequency and P <0.05 in AUC). Conclusions These results demonstrate visceral hypersensitivity and colonic dysmotility in a rat model of type 2 diabetes mellitus accompanied by weight loss.
AB - Background/Aims Abnormal visceral sensitivity and disordered motility are common in patients with diabetes mellitus. The purpose of the present study was to investigate whether visceral sensation and bowel motility were altered in a rat model of type 2 diabetes mellitus accompanied by weight loss. Methods A type 2 diabetic rat model in adulthood was developed by administrating streptozotocin (STZ; 90 mg/kg, i.p.) to neonatal rats. Eight weeks after STZ administration, rats with blood glucose level of 200 mg/dL or higher were selected and used as diabetic group (n = 35) in this study. Abdominal withdrawal reflex and arterial pulse rate were measured to examine visceral nociception induced by colorectal distension (0.1-1.0 mL). The amplitude, frequency, and area under the curve (AUC) of spontaneous phasic contractions of colonic circular muscles were recorded in vitro to examine colonic motility. Results STZ-treated diabetic rats gained significantly less weight for 8 weeks than control (P <0.01). Forty-eight percent of the diabetic rats showed enhanced visceral nociceptive response to colorectal distension. Diabetic rats did not differ from control rats in colorectal compliance. However, the frequency and AUC, not the amplitude, of colonic spontaneous contraction in vitro was significantly decreased in diabetic rats compared to control rats (P <0.01 in frequency and P <0.05 in AUC). Conclusions These results demonstrate visceral hypersensitivity and colonic dysmotility in a rat model of type 2 diabetes mellitus accompanied by weight loss.
KW - Abdominal withdrawal reflex
KW - Colonic dysmotility
KW - Colorectal distension
KW - Diabetes mellitus, type 2
KW - Visceral hypersensitivity
UR - http://www.scopus.com/inward/record.url?scp=84944684066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944684066&partnerID=8YFLogxK
U2 - 10.5056/jnm15058
DO - 10.5056/jnm15058
M3 - Article
AN - SCOPUS:84944684066
VL - 21
SP - 581
EP - 588
JO - Journal of Neurogastroenterology and Motility
JF - Journal of Neurogastroenterology and Motility
SN - 2093-0879
IS - 4
ER -