Visceral hypersensitivity and altered colonic motilty after subsidence of inflammation in a rat model of colitis

Jun-Ho La, Tae Wan Kim, Tae Sik Sung, Jeoung Woo Kang, Hyun Ju Kim, Il Suk Yang

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Aim: Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS, which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. Methods: Colitis was induced by intracolonic instillation of 4 % acetic acid in male Sprague-Dawley rats. The extent of inflammation was assessed by histological examination and myeloperoxidase (MPO) activity assay. After subsidence of colitis, the rats were subjected to rectal distension and restraint stress, then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. Results: At 2 days post-induction of colitis, the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity. At 7 days post-induction of colitis, the histological features and MPO activity returned to normal. The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance, and defecated more stools than control animals when under stress. Conclusion: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.

Original languageEnglish (US)
Pages (from-to)2791-2795
Number of pages5
JournalWorld Journal of Gastroenterology
Volume9
Issue number12
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Colitis
Irritable Bowel Syndrome
Hypersensitivity
Inflammation
Peroxidase
Abdominal Reflex
Animal Disease Models
Defecation
Acetic Acid
Compliance
Sprague Dawley Rats
Histology
Colon

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Visceral hypersensitivity and altered colonic motilty after subsidence of inflammation in a rat model of colitis. / La, Jun-Ho; Kim, Tae Wan; Sung, Tae Sik; Kang, Jeoung Woo; Kim, Hyun Ju; Yang, Il Suk.

In: World Journal of Gastroenterology, Vol. 9, No. 12, 12.2003, p. 2791-2795.

Research output: Contribution to journalArticle

La, Jun-Ho ; Kim, Tae Wan ; Sung, Tae Sik ; Kang, Jeoung Woo ; Kim, Hyun Ju ; Yang, Il Suk. / Visceral hypersensitivity and altered colonic motilty after subsidence of inflammation in a rat model of colitis. In: World Journal of Gastroenterology. 2003 ; Vol. 9, No. 12. pp. 2791-2795.
@article{0c1a79b71dc2469892d66f3fd1960948,
title = "Visceral hypersensitivity and altered colonic motilty after subsidence of inflammation in a rat model of colitis",
abstract = "Aim: Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS, which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. Methods: Colitis was induced by intracolonic instillation of 4 {\%} acetic acid in male Sprague-Dawley rats. The extent of inflammation was assessed by histological examination and myeloperoxidase (MPO) activity assay. After subsidence of colitis, the rats were subjected to rectal distension and restraint stress, then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. Results: At 2 days post-induction of colitis, the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity. At 7 days post-induction of colitis, the histological features and MPO activity returned to normal. The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance, and defecated more stools than control animals when under stress. Conclusion: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.",
author = "Jun-Ho La and Kim, {Tae Wan} and Sung, {Tae Sik} and Kang, {Jeoung Woo} and Kim, {Hyun Ju} and Yang, {Il Suk}",
year = "2003",
month = "12",
language = "English (US)",
volume = "9",
pages = "2791--2795",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "12",

}

TY - JOUR

T1 - Visceral hypersensitivity and altered colonic motilty after subsidence of inflammation in a rat model of colitis

AU - La, Jun-Ho

AU - Kim, Tae Wan

AU - Sung, Tae Sik

AU - Kang, Jeoung Woo

AU - Kim, Hyun Ju

AU - Yang, Il Suk

PY - 2003/12

Y1 - 2003/12

N2 - Aim: Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS, which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. Methods: Colitis was induced by intracolonic instillation of 4 % acetic acid in male Sprague-Dawley rats. The extent of inflammation was assessed by histological examination and myeloperoxidase (MPO) activity assay. After subsidence of colitis, the rats were subjected to rectal distension and restraint stress, then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. Results: At 2 days post-induction of colitis, the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity. At 7 days post-induction of colitis, the histological features and MPO activity returned to normal. The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance, and defecated more stools than control animals when under stress. Conclusion: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.

AB - Aim: Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS, which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. Methods: Colitis was induced by intracolonic instillation of 4 % acetic acid in male Sprague-Dawley rats. The extent of inflammation was assessed by histological examination and myeloperoxidase (MPO) activity assay. After subsidence of colitis, the rats were subjected to rectal distension and restraint stress, then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. Results: At 2 days post-induction of colitis, the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity. At 7 days post-induction of colitis, the histological features and MPO activity returned to normal. The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance, and defecated more stools than control animals when under stress. Conclusion: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.

UR - http://www.scopus.com/inward/record.url?scp=0346967115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346967115&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 2791

EP - 2795

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 12

ER -