Vitamin D elicits anti-inflammatory response, inhibits contractile- associated proteins, and modulates toll-like receptors in human myometrial cells

Chandrasekhar Thota, Takeisha Farmer, Robert E. Garfield, Ramkumar Menon, Ayman Al-Hendy

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Infection during pregnancy triggers inflammation, which can increase myometrial contractions and the risk of premature labor and delivery. In this study, we assessed the effects of vitamin D, an anti-inflammatory ligand on cytokines, chemokines, toll-like receptors, and contractile-associated proteins on immortalized human myometrial smooth muscle (UtSM) cells stimulated with lipopolysaccharide (LPS), a bacterial endotoxin, or interleukin (IL)-1β and measured Toll-like receptor (TLR)-10 expression in pregnant myometrial tissues. A superarray analysis revealed downregulation of the chemokines monocyte chemoattractant protein (MCP)-1, Chemokine (C-X-C motif) ligand (CXCL)-10, CXCL-11, and chemokine (C-X3-C motif) ligand (CX3CL)-1; the proinflammatory cytokines IL-13 and tumor necrosis factor (TNF)-α; the TLR-4 and -5 and triggering receptor expressed on myeloid cells (TREM)-2 and upregulation of the anti-inflammatory cytokine IL-10, as well as Toll interacting protein (TOLLIP) and TREM-1 in vitamin D-treated UtSM cells. In the presence of LPS, vitamin D caused dose-dependent decreases in the messenger RNA expression of MCP-1, IL-1β, IL-13, TNF-α, TLR-4, and TLR-5, the contractile-associated proteins connexin 43, the oxytocin receptor, and the prostaglandin receptor but caused increases in IL-10 and TLR-10 in UtSM cells. The TLR-10 expression was higher in human myometrial tissue obtained from women at term not in labor compared to labor. Vitamin D also attenuated IL-1β-induced MCP-1, IL-6, connexin 43, cyclooxygenase (COX)-2, and prostaglandin receptor expression. Western analysis showed that vitamin D decreased MCP-1, TLR-4, and connexin 43 in the presence of LPS and decreased connexin 43 in the presence of IL-1β. Our results suggest that vitamin D can potentially decrease infection-induced increases in cytokines and contractile-associated proteins in the myometrium.

Original languageEnglish (US)
Pages (from-to)463-475
Number of pages13
JournalReproductive Sciences
Volume20
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Contractile Proteins
Toll-Like Receptors
Vitamin D
Toll-Like Receptor 10
Connexin 43
Anti-Inflammatory Agents
Chemokine CCL2
Interleukin-1
Toll-Like Receptor 4
Toll-Like Receptor 5
Prostaglandin Receptors
Cytokines
Lipopolysaccharides
Interleukin-13
Myeloid Cells
Chemokines
Interleukin-10
Tumor Necrosis Factor-alpha
Chemokine CX3CL1
Receptor-Interacting Protein Serine-Threonine Kinases

Keywords

  • contractile-associated proteins
  • cytokines
  • myometrial smooth muscle cells
  • toll-like receptors
  • UtSM cells
  • vitamin D

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Vitamin D elicits anti-inflammatory response, inhibits contractile- associated proteins, and modulates toll-like receptors in human myometrial cells. / Thota, Chandrasekhar; Farmer, Takeisha; Garfield, Robert E.; Menon, Ramkumar; Al-Hendy, Ayman.

In: Reproductive Sciences, Vol. 20, No. 4, 04.2013, p. 463-475.

Research output: Contribution to journalArticle

Thota, Chandrasekhar ; Farmer, Takeisha ; Garfield, Robert E. ; Menon, Ramkumar ; Al-Hendy, Ayman. / Vitamin D elicits anti-inflammatory response, inhibits contractile- associated proteins, and modulates toll-like receptors in human myometrial cells. In: Reproductive Sciences. 2013 ; Vol. 20, No. 4. pp. 463-475.
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