Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS

S. Alagarsamy, K. M. Johnson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have previously shown that N-methyl-D-aspartate (NMDA) increases nitric oxide synthase (NOS) activity in rat frontal cortex; however, the actual mechanism of this activation has not been addressed. Tetrodotoxin (TTX; 0.05 μM) inhibited NMDA-activated NOS, suggesting that TTX-sensitive Na+ channels are interposed between the NMDA receptors and the NOS cellular compartment. The NMDA response was also blocked by voltage-dependent Ca2+ channel (VDCC) blockers including Cd2+, Co2+, funnel web spider toxin (FTX) and ω-Aga IVa, but not by nifedipine or ω-conotoxin. These data suggest that Ca2+ flux through P- and/or Q-type VDCC subsequent to NMDA-induced depolarization may be at least as important for NOS activation as Ca2+ entry through the NMDA receptor.

Original languageEnglish (US)
Pages (from-to)2250-2254
Number of pages5
JournalNeuroReport
Volume6
Issue number16
StatePublished - 1995

Fingerprint

N-Methylaspartate
Calcium Channels
Nitric Oxide Synthase
N-Methyl-D-Aspartate Receptors
Conotoxins
Tetrodotoxin
Frontal Lobe
Nifedipine
spider toxin FTX

Keywords

  • Aga IVa toxin
  • Cortex
  • Glutamate
  • L-methyl-D-aspartate (NMDA)
  • P-type calcium channel
  • Receptor binding

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alagarsamy, S., & Johnson, K. M. (1995). Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS. NeuroReport, 6(16), 2250-2254.

Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS. / Alagarsamy, S.; Johnson, K. M.

In: NeuroReport, Vol. 6, No. 16, 1995, p. 2250-2254.

Research output: Contribution to journalArticle

Alagarsamy, S & Johnson, KM 1995, 'Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS', NeuroReport, vol. 6, no. 16, pp. 2250-2254.
Alagarsamy, S. ; Johnson, K. M. / Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS. In: NeuroReport. 1995 ; Vol. 6, No. 16. pp. 2250-2254.
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