Voltage-dependent tetrodotoxin binding to single batrachotoxin-modified Na channels recorded from intact neuroblastoma cells

Duane R. McPherson, Li-Yen Huang

Research output: Contribution to journalArticle

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Abstract

To clarify the voltage-dependent actions of tetrodotoxin (TTX) on Na channels in cellular membranes, we examined the TTX block of single batrachotoxin-modified Na channels in neuroblastoma cells. We found these Na channels had a high affinity for TTX which decreased e-fold per 35.5 mV depolarization. The decrease in affinity resulted primarily from a decrease in the blocking rate for TTX; the unblocking rate increased slightly with depolarization. While the voltage-dependence of TTX binding to neuroblastoma Na channels was similar to that reported in purified Na channels incorporated in bilayers, the magnitude and voltage-dependence of the rate constants were quite different.

Original languageEnglish (US)
Pages (from-to)201-204
Number of pages4
JournalNeuroscience Letters
Volume131
Issue number2
DOIs
StatePublished - Oct 14 1991
Externally publishedYes

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Keywords

  • Neuroblastoma cell
  • Patchclamp
  • Single batrachotoxin-modified sodium channel
  • Sodium channel
  • Voltage-dependent tetrodotoxin binding

ASJC Scopus subject areas

  • Neuroscience(all)

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