VP4 Protein from Human Rhinovirus 14 Is Released by Pressure and Locked in the Capsid by the Antiviral Compound WIN

Rafael B. Gonçalves, Ygara S. Mendes, Marcia R. Soares, Umesh Katpally, Thomas Smith, Jerson L. Silva, Andréa C. Oliveira

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Rhinoviruses are the major causative agents of the common cold in humans. Here, we studied the stability of human rhinovirus type 14 (HRV14) under conditions of high hydrostatic pressure, low temperature, and urea in the absence and presence of an antiviral drug. Capsid dissociation and changes in the protein conformation were monitored by fluorescence spectroscopy, light scattering, circular dichroism, gel filtration chromatography, mass spectrometry and infectivity assays. The data show that high pressure induces the dissociation of HRV14 and that this process is inhibited by WIN 52084. MALDI-TOF mass spectrometry experiments demonstrate that VP4, the most internal viral protein, is released from the capsid by pressure treatment. This release of VP4 is concomitant with loss of infectivity. Our studies also show that at least one antiviral effect of the WIN drugs involves the locking of VP4 inside the capsid by blocking the dynamics associated with cell attachment.

Original languageEnglish (US)
Pages (from-to)295-306
Number of pages12
JournalJournal of Molecular Biology
Volume366
Issue number1
DOIs
StatePublished - Feb 9 2007
Externally publishedYes

Fingerprint

Rhinovirus
Capsid
Antiviral Agents
Pressure
Mass Spectrometry
Common Cold
Protein Conformation
Proteins
Hydrostatic Pressure
Fluorescence Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Viral Proteins
Circular Dichroism
Gel Chromatography
Urea
Light
Temperature
Pharmaceutical Preparations

Keywords

  • fluorescence
  • human rhinovirus
  • hydrostatic pressure
  • mass spectrometry
  • WIN drugs

ASJC Scopus subject areas

  • Virology

Cite this

VP4 Protein from Human Rhinovirus 14 Is Released by Pressure and Locked in the Capsid by the Antiviral Compound WIN. / Gonçalves, Rafael B.; Mendes, Ygara S.; Soares, Marcia R.; Katpally, Umesh; Smith, Thomas; Silva, Jerson L.; Oliveira, Andréa C.

In: Journal of Molecular Biology, Vol. 366, No. 1, 09.02.2007, p. 295-306.

Research output: Contribution to journalArticle

Gonçalves, Rafael B. ; Mendes, Ygara S. ; Soares, Marcia R. ; Katpally, Umesh ; Smith, Thomas ; Silva, Jerson L. ; Oliveira, Andréa C. / VP4 Protein from Human Rhinovirus 14 Is Released by Pressure and Locked in the Capsid by the Antiviral Compound WIN. In: Journal of Molecular Biology. 2007 ; Vol. 366, No. 1. pp. 295-306.
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