TY - JOUR
T1 - Water Soluble Fluorescent Graphene Nanodots
AU - Singh, Shikha
AU - Mitra, Kheyanath
AU - Senapati, Sudipta
AU - Singh, Rajshree
AU - Biswas, Yajnaseni
AU - Sen Gupta, Susanta K.
AU - Misra, Nira
AU - Mandal, Tarun K.
AU - Maiti, Pralay
AU - Ray, Biswajit
N1 - Funding Information:
BR acknowledges the financial support of DBT (Govt. of India) through grant no. BT/PR889/NNT/28/570/2011. SS acknowledge UGC-BSR [no. Chem.2015-16UGC-BSR/RFSMS/431] (Govt. of India) and CSIR-SRF [File no. 9/13(664)/2017-EMR-I] (Govt. of India) and KM acknowledge DBT (Govt. of India), for providing research fellowship. Authors also acknowledge Prof. A. K. Ghosh (Dept. of Physics, BHU) for providing poly (tetrafluoroethylene) (Teflon)-lined autoclave reactor.
Funding Information:
BR acknowledges the financial support of DBT (Govt. of India)through grant no. BT/PR889/NNT/28/570/2011. SS acknowledge UGC-BSR [no. Chem.2015-16UGC-BSR/RFSMS/431] (Govt. of India) and CSIR-SRF [File no. 9/13(664)/2017-EMR-I] (Govt. of India) and KM acknowledge DBT (Govt. of India), for providing research fellowship. Authors also acknowledge Prof. A. K. Ghosh (Dept. of Physics, BHU) for providing poly (tetrafluoroethylene) (Teflon)-lined autoclave reactor.
Publisher Copyright:
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/11
Y1 - 2018/11
N2 - Water soluble fluorescent graphene nanodots (GND) have been successfully prepared through a bottom up approach from brominated pyrene via alkaline hydrothermal condensation. FT-IR, UV-Vis, Raman, XRD, AFM, and TEM studies have shown the formation of −OH group rich nanodots having graphene features with an approximate size of 90 nm. Steady state and 3D fluorescence studies have shown efficient fluorescence emission with maximum at 540 nm upon excitation at 450 nm in pH≤7. Plasmid deoxyribonucleic acid (plasmid DNA) and bovine serum albumin (BSA) have shown their interaction with GND, which was studied via fluorescence quenching of GND and BSA, respectively. Selective quenching of the Förster resonance energy transfer (FRET) of BSA-GND conjugate via ascorbic acid (AA) has been used to construct a calibration curve for AA estimation with a linear range and limit of detection of 34.00–112.00 μmole/L and 31.343 μmole/L, respectively. GND has also shown concentration-dependent turn offchemosensing towards heavy metals like Pb2+, Cu2+, Hg2+ and Zn2+. In-vitromulticolor fluorescence imaging has been observed in GND-labelled HeLa (human cervical cancer) and NIH-3T3 (mouse embryonic fibroblast) cell lines with better uptake by HeLa cells. GND also has shown very high viability for both the cell lines up to the tested concentration of 300 μg/mL.
AB - Water soluble fluorescent graphene nanodots (GND) have been successfully prepared through a bottom up approach from brominated pyrene via alkaline hydrothermal condensation. FT-IR, UV-Vis, Raman, XRD, AFM, and TEM studies have shown the formation of −OH group rich nanodots having graphene features with an approximate size of 90 nm. Steady state and 3D fluorescence studies have shown efficient fluorescence emission with maximum at 540 nm upon excitation at 450 nm in pH≤7. Plasmid deoxyribonucleic acid (plasmid DNA) and bovine serum albumin (BSA) have shown their interaction with GND, which was studied via fluorescence quenching of GND and BSA, respectively. Selective quenching of the Förster resonance energy transfer (FRET) of BSA-GND conjugate via ascorbic acid (AA) has been used to construct a calibration curve for AA estimation with a linear range and limit of detection of 34.00–112.00 μmole/L and 31.343 μmole/L, respectively. GND has also shown concentration-dependent turn offchemosensing towards heavy metals like Pb2+, Cu2+, Hg2+ and Zn2+. In-vitromulticolor fluorescence imaging has been observed in GND-labelled HeLa (human cervical cancer) and NIH-3T3 (mouse embryonic fibroblast) cell lines with better uptake by HeLa cells. GND also has shown very high viability for both the cell lines up to the tested concentration of 300 μg/mL.
KW - bovine serum albumin
KW - brominated pyrene
KW - fluorescence
KW - Förster resonance energy transfer
KW - graphene nanodots
UR - http://www.scopus.com/inward/record.url?scp=85053287485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053287485&partnerID=8YFLogxK
U2 - 10.1002/cnma.201800285
DO - 10.1002/cnma.201800285
M3 - Article
AN - SCOPUS:85053287485
SN - 2199-692X
VL - 4
SP - 1177
EP - 1188
JO - ChemNanoMat
JF - ChemNanoMat
IS - 11
ER -