West nile virus infection in human and mouse cornea tissue

Bradley J. Blitvich, Tian Wang, Vandana Saxena, Shemin Zeng, Karen M. Harmon, Matthew D. Raymond, Kenneth M. Goins, Cynthia R. Reed, Robert F. Mullins, Mark A. Greiner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1-6 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 × 107 plaque-forming units (pfu)/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1-5 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 × 104 pfu/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.

Original languageEnglish (US)
Pages (from-to)1185-1191
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume95
Issue number5
DOIs
StatePublished - Nov 1 2016

Fingerprint

West Nile virus
Virus Diseases
Cornea
Viral RNA
Viral Antigens
Viruses
Virus Replication
Epithelial Cells

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Blitvich, B. J., Wang, T., Saxena, V., Zeng, S., Harmon, K. M., Raymond, M. D., ... Greiner, M. A. (2016). West nile virus infection in human and mouse cornea tissue. American Journal of Tropical Medicine and Hygiene, 95(5), 1185-1191. https://doi.org/10.4269/ajtmh.16-0256

West nile virus infection in human and mouse cornea tissue. / Blitvich, Bradley J.; Wang, Tian; Saxena, Vandana; Zeng, Shemin; Harmon, Karen M.; Raymond, Matthew D.; Goins, Kenneth M.; Reed, Cynthia R.; Mullins, Robert F.; Greiner, Mark A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 5, 01.11.2016, p. 1185-1191.

Research output: Contribution to journalArticle

Blitvich, BJ, Wang, T, Saxena, V, Zeng, S, Harmon, KM, Raymond, MD, Goins, KM, Reed, CR, Mullins, RF & Greiner, MA 2016, 'West nile virus infection in human and mouse cornea tissue', American Journal of Tropical Medicine and Hygiene, vol. 95, no. 5, pp. 1185-1191. https://doi.org/10.4269/ajtmh.16-0256
Blitvich, Bradley J. ; Wang, Tian ; Saxena, Vandana ; Zeng, Shemin ; Harmon, Karen M. ; Raymond, Matthew D. ; Goins, Kenneth M. ; Reed, Cynthia R. ; Mullins, Robert F. ; Greiner, Mark A. / West nile virus infection in human and mouse cornea tissue. In: American Journal of Tropical Medicine and Hygiene. 2016 ; Vol. 95, No. 5. pp. 1185-1191.
@article{589260f8502543cdbd4d54c9220db463,
title = "West nile virus infection in human and mouse cornea tissue",
abstract = "The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1-6 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 × 107 plaque-forming units (pfu)/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1-5 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 × 104 pfu/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.",
author = "Blitvich, {Bradley J.} and Tian Wang and Vandana Saxena and Shemin Zeng and Harmon, {Karen M.} and Raymond, {Matthew D.} and Goins, {Kenneth M.} and Reed, {Cynthia R.} and Mullins, {Robert F.} and Greiner, {Mark A.}",
year = "2016",
month = "11",
day = "1",
doi = "10.4269/ajtmh.16-0256",
language = "English (US)",
volume = "95",
pages = "1185--1191",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "5",

}

TY - JOUR

T1 - West nile virus infection in human and mouse cornea tissue

AU - Blitvich, Bradley J.

AU - Wang, Tian

AU - Saxena, Vandana

AU - Zeng, Shemin

AU - Harmon, Karen M.

AU - Raymond, Matthew D.

AU - Goins, Kenneth M.

AU - Reed, Cynthia R.

AU - Mullins, Robert F.

AU - Greiner, Mark A.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1-6 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 × 107 plaque-forming units (pfu)/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1-5 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 × 104 pfu/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.

AB - The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1-6 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 × 107 plaque-forming units (pfu)/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1-5 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 × 104 pfu/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.

UR - http://www.scopus.com/inward/record.url?scp=84994275123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994275123&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.16-0256

DO - 10.4269/ajtmh.16-0256

M3 - Article

C2 - 27672204

AN - SCOPUS:84994275123

VL - 95

SP - 1185

EP - 1191

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 5

ER -