West Nile virus infection of Drosophila melanogaster induces a protective RNAi response

Heather L. Chotkowski, Alexander T. Ciota, Yongqing Jia, Francesc Puig-Basagoiti, Laura D. Kramer, Pei-Yong Shi, Robert L. Glaser

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of ~ 25 nt WNV-homologous small RNAs was detected in infected S2 cells but not C6/36 cells. RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous small RNAs produced in infected S2 cells are functional small interfering RNAs. WNV was shown to infect adult D. melanogaster, and adult flies containing mutations in each of four different RNAi genes (Argonaute 2, spindle-E, piwi, and Dicer-2) were significantly more susceptible to WNV infection than wildtype flies. These results combined with the analysis of WNV infection of S2 and C6/36 cells support the conclusion that WNV infection of D. melanogaster, but perhaps not Ae. albopictus, induces a protective RNAi response.

Original languageEnglish (US)
Pages (from-to)197-206
Number of pages10
JournalVirology
Volume377
Issue number1
DOIs
StatePublished - Jul 20 2008
Externally publishedYes

Fingerprint

West Nile virus
Virus Diseases
RNA Interference
Drosophila melanogaster
RNA
Diptera
Aedes
Small Interfering RNA
Insects

Keywords

  • Drosophila
  • Innate immunity
  • RNAi
  • West Nile virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Chotkowski, H. L., Ciota, A. T., Jia, Y., Puig-Basagoiti, F., Kramer, L. D., Shi, P-Y., & Glaser, R. L. (2008). West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. Virology, 377(1), 197-206. https://doi.org/10.1016/j.virol.2008.04.021

West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. / Chotkowski, Heather L.; Ciota, Alexander T.; Jia, Yongqing; Puig-Basagoiti, Francesc; Kramer, Laura D.; Shi, Pei-Yong; Glaser, Robert L.

In: Virology, Vol. 377, No. 1, 20.07.2008, p. 197-206.

Research output: Contribution to journalArticle

Chotkowski, HL, Ciota, AT, Jia, Y, Puig-Basagoiti, F, Kramer, LD, Shi, P-Y & Glaser, RL 2008, 'West Nile virus infection of Drosophila melanogaster induces a protective RNAi response', Virology, vol. 377, no. 1, pp. 197-206. https://doi.org/10.1016/j.virol.2008.04.021
Chotkowski HL, Ciota AT, Jia Y, Puig-Basagoiti F, Kramer LD, Shi P-Y et al. West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. Virology. 2008 Jul 20;377(1):197-206. https://doi.org/10.1016/j.virol.2008.04.021
Chotkowski, Heather L. ; Ciota, Alexander T. ; Jia, Yongqing ; Puig-Basagoiti, Francesc ; Kramer, Laura D. ; Shi, Pei-Yong ; Glaser, Robert L. / West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. In: Virology. 2008 ; Vol. 377, No. 1. pp. 197-206.
@article{6b67be3a2d7943338ddba2bb540544dc,
title = "West Nile virus infection of Drosophila melanogaster induces a protective RNAi response",
abstract = "To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of ~ 25 nt WNV-homologous small RNAs was detected in infected S2 cells but not C6/36 cells. RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous small RNAs produced in infected S2 cells are functional small interfering RNAs. WNV was shown to infect adult D. melanogaster, and adult flies containing mutations in each of four different RNAi genes (Argonaute 2, spindle-E, piwi, and Dicer-2) were significantly more susceptible to WNV infection than wildtype flies. These results combined with the analysis of WNV infection of S2 and C6/36 cells support the conclusion that WNV infection of D. melanogaster, but perhaps not Ae. albopictus, induces a protective RNAi response.",
keywords = "Drosophila, Innate immunity, RNAi, West Nile virus",
author = "Chotkowski, {Heather L.} and Ciota, {Alexander T.} and Yongqing Jia and Francesc Puig-Basagoiti and Kramer, {Laura D.} and Pei-Yong Shi and Glaser, {Robert L.}",
year = "2008",
month = "7",
day = "20",
doi = "10.1016/j.virol.2008.04.021",
language = "English (US)",
volume = "377",
pages = "197--206",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - West Nile virus infection of Drosophila melanogaster induces a protective RNAi response

AU - Chotkowski, Heather L.

AU - Ciota, Alexander T.

AU - Jia, Yongqing

AU - Puig-Basagoiti, Francesc

AU - Kramer, Laura D.

AU - Shi, Pei-Yong

AU - Glaser, Robert L.

PY - 2008/7/20

Y1 - 2008/7/20

N2 - To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of ~ 25 nt WNV-homologous small RNAs was detected in infected S2 cells but not C6/36 cells. RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous small RNAs produced in infected S2 cells are functional small interfering RNAs. WNV was shown to infect adult D. melanogaster, and adult flies containing mutations in each of four different RNAi genes (Argonaute 2, spindle-E, piwi, and Dicer-2) were significantly more susceptible to WNV infection than wildtype flies. These results combined with the analysis of WNV infection of S2 and C6/36 cells support the conclusion that WNV infection of D. melanogaster, but perhaps not Ae. albopictus, induces a protective RNAi response.

AB - To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of ~ 25 nt WNV-homologous small RNAs was detected in infected S2 cells but not C6/36 cells. RNAi knockdown of Argonaute 2 in S2 cells resulted in slightly increased susceptibility to WNV infection, suggesting that some WNV-homologous small RNAs produced in infected S2 cells are functional small interfering RNAs. WNV was shown to infect adult D. melanogaster, and adult flies containing mutations in each of four different RNAi genes (Argonaute 2, spindle-E, piwi, and Dicer-2) were significantly more susceptible to WNV infection than wildtype flies. These results combined with the analysis of WNV infection of S2 and C6/36 cells support the conclusion that WNV infection of D. melanogaster, but perhaps not Ae. albopictus, induces a protective RNAi response.

KW - Drosophila

KW - Innate immunity

KW - RNAi

KW - West Nile virus

UR - http://www.scopus.com/inward/record.url?scp=44949256129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949256129&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2008.04.021

DO - 10.1016/j.virol.2008.04.021

M3 - Article

C2 - 18501400

AN - SCOPUS:44949256129

VL - 377

SP - 197

EP - 206

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -