West nile virus infection of primary mouse neuronal and neuroglial cells: The role of astrocytes in chronic infection

Jose A P Diniz, Amelia P A Travassos Da Rosa, Hilda Guzman, Fangling Xu, Shu Yuan Xiao, Vsevolod Popov, Pedro F C Vasconcelos, Robert B. Tesh

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Primary cultures of embryonic murine neurons and newborn mouse astrocytes were inoculated with West Nile virus (WNV) strain NY385-99 to compare the pathogenesis of WNV infection in these types of CNS cells. Two different outcomes were observed. WNV infection in the neurons was rapidly progressive and destructive; within 5 days, all of the neurons were destroyed through apoptosis. WNV infection in the astrocytes evolved more slowly and did not seem to be highly lethal to the cells. The infected astrocytes continued to produce infectious virus (104.6-106.5 PFU/mL) for 114 days, in a permissive, persistent infection. During this period, WNV antigen could be shown in the cytoplasm of the infected astrocytes by immunocytochemical assay, transmission electron microscopy of ultrathin sections, and in the cell culture medium by complement fixation test. Our results with this in vitro experimental murine cell model indicate that astrocytes can develop chronic or persistent infection with WNV, suggesting that these cells may play a role in the maintenance of WNV in the CNS.

Original languageEnglish (US)
Pages (from-to)691-696
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume75
Issue number4
StatePublished - 2006
Externally publishedYes

Fingerprint

West Nile virus
Virus Diseases
Neuroglia
Astrocytes
Infection
Neurons
Complement Fixation Tests
Transmission Electron Microscopy
Culture Media
Cytoplasm
Cell Culture Techniques
Maintenance
Apoptosis
Viruses
Antigens

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Diniz, J. A. P., Travassos Da Rosa, A. P. A., Guzman, H., Xu, F., Xiao, S. Y., Popov, V., ... Tesh, R. B. (2006). West nile virus infection of primary mouse neuronal and neuroglial cells: The role of astrocytes in chronic infection. American Journal of Tropical Medicine and Hygiene, 75(4), 691-696.

West nile virus infection of primary mouse neuronal and neuroglial cells : The role of astrocytes in chronic infection. / Diniz, Jose A P; Travassos Da Rosa, Amelia P A; Guzman, Hilda; Xu, Fangling; Xiao, Shu Yuan; Popov, Vsevolod; Vasconcelos, Pedro F C; Tesh, Robert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 75, No. 4, 2006, p. 691-696.

Research output: Contribution to journalArticle

Diniz, JAP, Travassos Da Rosa, APA, Guzman, H, Xu, F, Xiao, SY, Popov, V, Vasconcelos, PFC & Tesh, RB 2006, 'West nile virus infection of primary mouse neuronal and neuroglial cells: The role of astrocytes in chronic infection', American Journal of Tropical Medicine and Hygiene, vol. 75, no. 4, pp. 691-696.
Diniz, Jose A P ; Travassos Da Rosa, Amelia P A ; Guzman, Hilda ; Xu, Fangling ; Xiao, Shu Yuan ; Popov, Vsevolod ; Vasconcelos, Pedro F C ; Tesh, Robert B. / West nile virus infection of primary mouse neuronal and neuroglial cells : The role of astrocytes in chronic infection. In: American Journal of Tropical Medicine and Hygiene. 2006 ; Vol. 75, No. 4. pp. 691-696.
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