West Nile virus infection of the placenta

Justin G. Julander, Quinton A. Winger, Lee F. Rickords, Pei-Yong Shi, Mark Tilgner, Iwona Binduga-Gajewska, Robert W. Sidwell, John D. Morrey

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Intrauterine infection of fetuses with West Nile virus (WNV) has been implicated in cases of women infected during pregnancy. Infection of timed-pregnant mice on 5.5, 7.5, and 9.5 days post-coitus (dpc) resulted in fetal infection. Infection of dams on 11.5 and 14.5 dpc resulted in little and no fetal infection, respectively. Pre-implantation embryos in culture were also infected with WNV after the blastocyst stage and the formation of trophectoderm. Green fluorescent protein (GFP) expression was observed in a trophoblast stem (TS) cell line after infection with a GFP-expressing WNV construct. However, no fluorescence was observed in differentiated trophoblast giant cell (TGC) cultures. GFP fluorescence was present in TGC cultures if infected TS cells were induced to differentiate. These results suggest that embryos are susceptible to WNV infection after the formation of the trophectoderm around 3.5 dpc through the formation of the functional placenta around 10.5 dpc.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalVirology
Volume347
Issue number1
DOIs
StatePublished - Mar 30 2006
Externally publishedYes

Fingerprint

West Nile virus
Virus Diseases
Placenta
Coitus
Trophoblasts
Green Fluorescent Proteins
Infection
Giant Cells
Stem Cells
Cell Culture Techniques
Fluorescence
Blastocyst
Fetus
Embryonic Structures
Cell Line
Pregnancy

Keywords

  • Intrauterine
  • Placenta
  • Pregnancy
  • Trophoblast
  • Vertical transmission
  • West Nile virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Julander, J. G., Winger, Q. A., Rickords, L. F., Shi, P-Y., Tilgner, M., Binduga-Gajewska, I., ... Morrey, J. D. (2006). West Nile virus infection of the placenta. Virology, 347(1), 175-182. https://doi.org/10.1016/j.virol.2005.11.040

West Nile virus infection of the placenta. / Julander, Justin G.; Winger, Quinton A.; Rickords, Lee F.; Shi, Pei-Yong; Tilgner, Mark; Binduga-Gajewska, Iwona; Sidwell, Robert W.; Morrey, John D.

In: Virology, Vol. 347, No. 1, 30.03.2006, p. 175-182.

Research output: Contribution to journalArticle

Julander, JG, Winger, QA, Rickords, LF, Shi, P-Y, Tilgner, M, Binduga-Gajewska, I, Sidwell, RW & Morrey, JD 2006, 'West Nile virus infection of the placenta', Virology, vol. 347, no. 1, pp. 175-182. https://doi.org/10.1016/j.virol.2005.11.040
Julander JG, Winger QA, Rickords LF, Shi P-Y, Tilgner M, Binduga-Gajewska I et al. West Nile virus infection of the placenta. Virology. 2006 Mar 30;347(1):175-182. https://doi.org/10.1016/j.virol.2005.11.040
Julander, Justin G. ; Winger, Quinton A. ; Rickords, Lee F. ; Shi, Pei-Yong ; Tilgner, Mark ; Binduga-Gajewska, Iwona ; Sidwell, Robert W. ; Morrey, John D. / West Nile virus infection of the placenta. In: Virology. 2006 ; Vol. 347, No. 1. pp. 175-182.
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