Western equine encephalitis virus: Evolutionary analysis of a declining alphavirus based on complete genome sequences

Nicholas A. Bergren, Albert J. Auguste, Naomi L. Forrester, Surendra S. Negi, Werner Braun, Scott Weaver

Research output: Contribution to journalArticle

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Abstract

Western equine encephalitis virus (WEEV) is an arbovirus from the genus Alphavirus, family Togaviridae, which circulates in North America between birds and mosquitoes, occasionally causing disease in humans and equids. In recent decades, human infection has decreased dramatically; the last documented human case in North America occurred in 1994, and the virus has not been detected in mosquito pools since 2008. Because limited information exists regarding the evolution of WEEV, we analyzed the genomic sequences of 33 low-passage-number strains with diverse geographic and temporal distributions and performed comprehensive phylogenetic analyses. Our results indicated that WEEV is a highly conserved alphavirus with only approximately 5% divergence in its most variable genes. We confirmed the presence of the previously determined group A and B lineages and further resolved group B into three sublineages. We also observed an increase in relative genetic diversity during the mid- 20th century, which correlates with the emergence and cocirculation of several group B sublineages. The estimated WEEV population size dropped in the 1990s, with only the group B3 lineage being sampled in the past 20 years. Structural mapping showed that the majority of substitutions in the envelope glycoproteins occurred at the E2-E2 interface. We hypothesize that an event occurred in the mid-20th century that resulted in the increased genetic diversity of WEEV in North America, followed by genetic constriction due to either competitive displacement by the B3 sublineage or stochastic events resulting from a population decline.

Original languageEnglish
Pages (from-to)9260-9267
Number of pages8
JournalJournal of Virology
Volume88
Issue number16
DOIs
StatePublished - 2014

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Western Equine Encephalitis Viruses
Western equine encephalitis virus
Alphavirus
Genome
North America
genome
Culicidae
Togaviridae
Arboviruses
arboviruses
genetic variation
Population Density
human diseases
Constriction
Birds
eclosion
glycoproteins
Glycoproteins
population size
Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

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Western equine encephalitis virus : Evolutionary analysis of a declining alphavirus based on complete genome sequences. / Bergren, Nicholas A.; Auguste, Albert J.; Forrester, Naomi L.; Negi, Surendra S.; Braun, Werner; Weaver, Scott.

In: Journal of Virology, Vol. 88, No. 16, 2014, p. 9260-9267.

Research output: Contribution to journalArticle

Bergren, Nicholas A. ; Auguste, Albert J. ; Forrester, Naomi L. ; Negi, Surendra S. ; Braun, Werner ; Weaver, Scott. / Western equine encephalitis virus : Evolutionary analysis of a declining alphavirus based on complete genome sequences. In: Journal of Virology. 2014 ; Vol. 88, No. 16. pp. 9260-9267.
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