Whey protein ingestion in elderly persons results in greater muscle protein accrual than ingestion of its constituent essential amino acid content

Christos S. Katsanos, David L. Chinkes, Douglas Paddon-Jones, Xiao jun Zhang, Asle Aarsland, Robert R. Wolfe

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

It is recognized that both whey protein (WY) and essential amino acids (EAA) are stimuli for muscle protein anabolism. The aim of the present study was to determine if the effects of WY ingestion on muscle protein accrual in elderly persons are due solely to its constituent EAA content. Fifteen elderly persons were randomly assigned to ingest a bolus of either 15 g of WY, 6.72 g of EAA, or 7.57 g of nonessential amino acids (NEAA). We used the leg arteriovenous model to measure the leg phenylalanine balance, which is an index of muscle protein accrual. Phenylalanine balance (nmol{bullet operator}min-1{bullet operator}kg lean leg mass-1) during the 3.5 hours after the bolus ingestion improved in the WY (-216 ± 14 vs -105 ± 19; P < .05) but not in the EAA (-203 ± 21 vs -172 ± 38; P > .05) or NEAA groups (-203 ± 19 vs -204 ± 21; P > .05). The insulin response (uIU{bullet operator}mL-1{bullet operator}210 min-1) during the same period was lower in both the NEAA (48 ± 40) and EAA (213 ± 127) when compared to the WY (1073 ± 229; P < .05). In conclusion, WY ingestion improves skeletal muscle protein accrual through mechanisms that are beyond those attributed to its EAA content. This finding may have practical implications for the formulation of nutritional supplements to enhance muscle anabolism in older individuals.

Original languageEnglish (US)
Pages (from-to)651-658
Number of pages8
JournalNutrition Research
Volume28
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Essential Amino Acids
Muscle Proteins
Eating
Leg
Phenylalanine
Amino Acids
Whey Proteins
Skeletal Muscle
Insulin
Muscles

Keywords

  • EAA
  • essential amino acids
  • Free amino acids
  • GCRC
  • General Clinical Research Center
  • GIP
  • glucose-dependent insulinotropic polypeptide
  • Humans
  • Intact protein
  • NEAA
  • nonessential amino acids
  • PB
  • phenylalanine balance
  • Protein balance
  • Protein metabolism
  • Protein supplement
  • whey protein
  • WY

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Whey protein ingestion in elderly persons results in greater muscle protein accrual than ingestion of its constituent essential amino acid content. / Katsanos, Christos S.; Chinkes, David L.; Paddon-Jones, Douglas; Zhang, Xiao jun; Aarsland, Asle; Wolfe, Robert R.

In: Nutrition Research, Vol. 28, No. 10, 10.2008, p. 651-658.

Research output: Contribution to journalArticle

Katsanos, Christos S. ; Chinkes, David L. ; Paddon-Jones, Douglas ; Zhang, Xiao jun ; Aarsland, Asle ; Wolfe, Robert R. / Whey protein ingestion in elderly persons results in greater muscle protein accrual than ingestion of its constituent essential amino acid content. In: Nutrition Research. 2008 ; Vol. 28, No. 10. pp. 651-658.
@article{c7367754c9814062bfda07005ae16acf,
title = "Whey protein ingestion in elderly persons results in greater muscle protein accrual than ingestion of its constituent essential amino acid content",
abstract = "It is recognized that both whey protein (WY) and essential amino acids (EAA) are stimuli for muscle protein anabolism. The aim of the present study was to determine if the effects of WY ingestion on muscle protein accrual in elderly persons are due solely to its constituent EAA content. Fifteen elderly persons were randomly assigned to ingest a bolus of either 15 g of WY, 6.72 g of EAA, or 7.57 g of nonessential amino acids (NEAA). We used the leg arteriovenous model to measure the leg phenylalanine balance, which is an index of muscle protein accrual. Phenylalanine balance (nmol{bullet operator}min-1{bullet operator}kg lean leg mass-1) during the 3.5 hours after the bolus ingestion improved in the WY (-216 ± 14 vs -105 ± 19; P < .05) but not in the EAA (-203 ± 21 vs -172 ± 38; P > .05) or NEAA groups (-203 ± 19 vs -204 ± 21; P > .05). The insulin response (uIU{bullet operator}mL-1{bullet operator}210 min-1) during the same period was lower in both the NEAA (48 ± 40) and EAA (213 ± 127) when compared to the WY (1073 ± 229; P < .05). In conclusion, WY ingestion improves skeletal muscle protein accrual through mechanisms that are beyond those attributed to its EAA content. This finding may have practical implications for the formulation of nutritional supplements to enhance muscle anabolism in older individuals.",
keywords = "EAA, essential amino acids, Free amino acids, GCRC, General Clinical Research Center, GIP, glucose-dependent insulinotropic polypeptide, Humans, Intact protein, NEAA, nonessential amino acids, PB, phenylalanine balance, Protein balance, Protein metabolism, Protein supplement, whey protein, WY",
author = "Katsanos, {Christos S.} and Chinkes, {David L.} and Douglas Paddon-Jones and Zhang, {Xiao jun} and Asle Aarsland and Wolfe, {Robert R.}",
year = "2008",
month = "10",
doi = "10.1016/j.nutres.2008.06.007",
language = "English (US)",
volume = "28",
pages = "651--658",
journal = "Nutrition Research",
issn = "0271-5317",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Whey protein ingestion in elderly persons results in greater muscle protein accrual than ingestion of its constituent essential amino acid content

AU - Katsanos, Christos S.

AU - Chinkes, David L.

AU - Paddon-Jones, Douglas

AU - Zhang, Xiao jun

AU - Aarsland, Asle

AU - Wolfe, Robert R.

PY - 2008/10

Y1 - 2008/10

N2 - It is recognized that both whey protein (WY) and essential amino acids (EAA) are stimuli for muscle protein anabolism. The aim of the present study was to determine if the effects of WY ingestion on muscle protein accrual in elderly persons are due solely to its constituent EAA content. Fifteen elderly persons were randomly assigned to ingest a bolus of either 15 g of WY, 6.72 g of EAA, or 7.57 g of nonessential amino acids (NEAA). We used the leg arteriovenous model to measure the leg phenylalanine balance, which is an index of muscle protein accrual. Phenylalanine balance (nmol{bullet operator}min-1{bullet operator}kg lean leg mass-1) during the 3.5 hours after the bolus ingestion improved in the WY (-216 ± 14 vs -105 ± 19; P < .05) but not in the EAA (-203 ± 21 vs -172 ± 38; P > .05) or NEAA groups (-203 ± 19 vs -204 ± 21; P > .05). The insulin response (uIU{bullet operator}mL-1{bullet operator}210 min-1) during the same period was lower in both the NEAA (48 ± 40) and EAA (213 ± 127) when compared to the WY (1073 ± 229; P < .05). In conclusion, WY ingestion improves skeletal muscle protein accrual through mechanisms that are beyond those attributed to its EAA content. This finding may have practical implications for the formulation of nutritional supplements to enhance muscle anabolism in older individuals.

AB - It is recognized that both whey protein (WY) and essential amino acids (EAA) are stimuli for muscle protein anabolism. The aim of the present study was to determine if the effects of WY ingestion on muscle protein accrual in elderly persons are due solely to its constituent EAA content. Fifteen elderly persons were randomly assigned to ingest a bolus of either 15 g of WY, 6.72 g of EAA, or 7.57 g of nonessential amino acids (NEAA). We used the leg arteriovenous model to measure the leg phenylalanine balance, which is an index of muscle protein accrual. Phenylalanine balance (nmol{bullet operator}min-1{bullet operator}kg lean leg mass-1) during the 3.5 hours after the bolus ingestion improved in the WY (-216 ± 14 vs -105 ± 19; P < .05) but not in the EAA (-203 ± 21 vs -172 ± 38; P > .05) or NEAA groups (-203 ± 19 vs -204 ± 21; P > .05). The insulin response (uIU{bullet operator}mL-1{bullet operator}210 min-1) during the same period was lower in both the NEAA (48 ± 40) and EAA (213 ± 127) when compared to the WY (1073 ± 229; P < .05). In conclusion, WY ingestion improves skeletal muscle protein accrual through mechanisms that are beyond those attributed to its EAA content. This finding may have practical implications for the formulation of nutritional supplements to enhance muscle anabolism in older individuals.

KW - EAA

KW - essential amino acids

KW - Free amino acids

KW - GCRC

KW - General Clinical Research Center

KW - GIP

KW - glucose-dependent insulinotropic polypeptide

KW - Humans

KW - Intact protein

KW - NEAA

KW - nonessential amino acids

KW - PB

KW - phenylalanine balance

KW - Protein balance

KW - Protein metabolism

KW - Protein supplement

KW - whey protein

KW - WY

UR - http://www.scopus.com/inward/record.url?scp=53249087926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53249087926&partnerID=8YFLogxK

U2 - 10.1016/j.nutres.2008.06.007

DO - 10.1016/j.nutres.2008.06.007

M3 - Article

C2 - 19083472

AN - SCOPUS:53249087926

VL - 28

SP - 651

EP - 658

JO - Nutrition Research

JF - Nutrition Research

SN - 0271-5317

IS - 10

ER -