Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan

Tsuneyuki Masuda, Fujiko Sunaga, Yuki Naoi, Mika Ito, Hiroki Takagi, Yukie Katayama, Tsutomu Omatsu, Mami Oba, Shoichi Sakaguchi, Tetsuya Furuya, Hiroshi Yamasato, Junsuke Shirai, Shinji Makino, Tetsuya Mizutani, Makoto Nagai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel virus related to the Enterovirus/Sapelovirus supergroup in the family Picornaviridae was identified in healthy porcine feces in Japan by using a metagenomics approach. The genome of the virus, named Sapelo-like porcine picornavirus Japan (SPPVJ) Pig/Isi-Im1/JPN/2016, had a type-IV internal ribosomal entry site and carried a 6978-nucleotide-long single open reading frame encoding a 2326 amino acids (aa) polyprotein precursor. The coding sequence region consisted of leader protein (68 aa), a structural protein region P1 (824 aa), and the non-structural protein regions P2 (672 aa) and P3 (762 aa). Among representative picornaviruses, the P1, 2C, and 3CD regions of SPPVJ had the highest aa identities of 64.4%, 61.9%, and 73.3%, respectively, with the corresponding regions of sapelo-like bat picornavirus BtVs-PicoV/SC2013. Sequencing analysis of the RT-PCR products derived from the 5’ untranslated and 3D regions revealed the presence of SPPVJ in 17.8% (19/107) of the feces from healthy and diarrheal pigs in 12 farms in 2015–2016. Further studies are needed to determine the origin and pathogenic potential of SPPJV in pigs and other mammals.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalVirus Research
Volume257
DOIs
StatePublished - Sep 15 2018

Fingerprint

Picornaviridae
Enterovirus
Feces
Japan
Swine
Genome
Amino Acids
Viruses
Polyproteins
Metagenomics
Proteins
5' Untranslated Regions
Open Reading Frames
Mammals
Nucleotides
Polymerase Chain Reaction

Keywords

  • Complete genome
  • Enterovirus/Sapelovirus supergroup
  • Novel picornavirus
  • Porcine feces

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan. / Masuda, Tsuneyuki; Sunaga, Fujiko; Naoi, Yuki; Ito, Mika; Takagi, Hiroki; Katayama, Yukie; Omatsu, Tsutomu; Oba, Mami; Sakaguchi, Shoichi; Furuya, Tetsuya; Yamasato, Hiroshi; Shirai, Junsuke; Makino, Shinji; Mizutani, Tetsuya; Nagai, Makoto.

In: Virus Research, Vol. 257, 15.09.2018, p. 68-73.

Research output: Contribution to journalArticle

Masuda, T, Sunaga, F, Naoi, Y, Ito, M, Takagi, H, Katayama, Y, Omatsu, T, Oba, M, Sakaguchi, S, Furuya, T, Yamasato, H, Shirai, J, Makino, S, Mizutani, T & Nagai, M 2018, 'Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan', Virus Research, vol. 257, pp. 68-73. https://doi.org/10.1016/j.virusres.2018.09.003
Masuda, Tsuneyuki ; Sunaga, Fujiko ; Naoi, Yuki ; Ito, Mika ; Takagi, Hiroki ; Katayama, Yukie ; Omatsu, Tsutomu ; Oba, Mami ; Sakaguchi, Shoichi ; Furuya, Tetsuya ; Yamasato, Hiroshi ; Shirai, Junsuke ; Makino, Shinji ; Mizutani, Tetsuya ; Nagai, Makoto. / Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan. In: Virus Research. 2018 ; Vol. 257. pp. 68-73.
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AU - Takagi, Hiroki

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