Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements

Kenneth A. Stapleford, Gonzalo Moratorio, Rasmus Henningsson, Rubing Chen, Séverine Matheus, Antoine Enfissi, Daphna Weissglas-Volkov, Ofer Isakov, Hervé Blanc, Bryan C. Mounce, Myrielle Dupont-Rouzeyrol, Noam Shomron, Scott Weaver, Magnus Fontes, Dominique Rousset, Marco Vignuzzi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Chikungunya virus (CHIKV), an alphavirus and member of the Togaviridae family, is capable of causing severe febrile disease in humans. In December of 2013 the Asian Lineage of CHIKV spread from the Old World to the Americas, spreading rapidly throughout the New World. Given this new emergence in naïve populations we studied the viral genetic diversity present in infected individuals to understand how CHIKV may have evolved during this continuing outbreak. Methodology/Principle Findings: We used deep-sequencing technologies coupled with well-established bioinformatics pipelines to characterize the minority variants and diversity present in CHIKV infected individuals from Guadeloupe and Martinique, two islands in the center of the epidemic. We observed changes in the consensus sequence as well as a diverse range of minority variants present at various levels in the population. Furthermore, we found that overall diversity was dramatically reduced after single passages in cell lines. Finally, we constructed an infectious clone from this outbreak and identified a novel 3’ untranslated region (UTR) structure, not previously found in nature, that led to increased replication in insect cells. Conclusions/Significance: Here we preformed an intrahost quasispecies analysis of the new CHIKV outbreak in the Caribbean. We identified novel variants present in infected individuals, as well as a new 3’UTR structure, suggesting that CHIKV has rapidly evolved in a short period of time once it entered this naïve population. These studies highlight the need to continue viral diversity surveillance over time as this epidemic evolves in order to understand the evolutionary potential of CHIKV.

Original languageEnglish (US)
Article numbere0004402
JournalPLoS Neglected Tropical Diseases
Volume10
Issue number1
DOIs
StatePublished - Jan 25 2016

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Chikungunya virus
Disease Outbreaks
Genome
Togaviridae
Martinique
Guadeloupe
Population
Alphavirus
High-Throughput Nucleotide Sequencing
Consensus Sequence
3' Untranslated Regions
Computational Biology
Islands
Insects
Fever
Clone Cells
Technology
Cell Line

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Stapleford, K. A., Moratorio, G., Henningsson, R., Chen, R., Matheus, S., Enfissi, A., ... Vignuzzi, M. (2016). Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements. PLoS Neglected Tropical Diseases, 10(1), [e0004402]. https://doi.org/10.1371/journal.pntd.0004402

Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements. / Stapleford, Kenneth A.; Moratorio, Gonzalo; Henningsson, Rasmus; Chen, Rubing; Matheus, Séverine; Enfissi, Antoine; Weissglas-Volkov, Daphna; Isakov, Ofer; Blanc, Hervé; Mounce, Bryan C.; Dupont-Rouzeyrol, Myrielle; Shomron, Noam; Weaver, Scott; Fontes, Magnus; Rousset, Dominique; Vignuzzi, Marco.

In: PLoS Neglected Tropical Diseases, Vol. 10, No. 1, e0004402, 25.01.2016.

Research output: Contribution to journalArticle

Stapleford, KA, Moratorio, G, Henningsson, R, Chen, R, Matheus, S, Enfissi, A, Weissglas-Volkov, D, Isakov, O, Blanc, H, Mounce, BC, Dupont-Rouzeyrol, M, Shomron, N, Weaver, S, Fontes, M, Rousset, D & Vignuzzi, M 2016, 'Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements', PLoS Neglected Tropical Diseases, vol. 10, no. 1, e0004402. https://doi.org/10.1371/journal.pntd.0004402
Stapleford, Kenneth A. ; Moratorio, Gonzalo ; Henningsson, Rasmus ; Chen, Rubing ; Matheus, Séverine ; Enfissi, Antoine ; Weissglas-Volkov, Daphna ; Isakov, Ofer ; Blanc, Hervé ; Mounce, Bryan C. ; Dupont-Rouzeyrol, Myrielle ; Shomron, Noam ; Weaver, Scott ; Fontes, Magnus ; Rousset, Dominique ; Vignuzzi, Marco. / Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements. In: PLoS Neglected Tropical Diseases. 2016 ; Vol. 10, No. 1.
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