Wing morphometry and genetic variability between culex coronator and culex usquatus (Diptera: Culicidae), two sibling species of the coronator group

Bruna Demari-Silva, Laura Cristina Multini, Lincoln Suesdek, Tatiane M.P. Oliveira, Maria Anice Mureb Sallum, Mauro Toledo Marrelli

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Culex coronator Dyar and Knab and Culex usquatus Dyar belong to the Coronator Group of the subgenus Culex. Culex coronator and Cx. usquatus are widespread and sympatric throughout their distribution range, which includes Brazil. Morphological identification of these species is based primarily on the characteristics of the male genitalia; females are indistinguishable using the qualitative characteristics employed in identification keys. The primary objective of this study was to distinguish females of Cx. coronator from those of Cx. usquatus employing both wing geometric morphometrics, and DNA sequences (NADH5, COI, Hunchback, and CAD). Additionally, we employed the isolation with migration model (IMa) to evaluate: 1) the migration rates and 2) the divergence time, between Cx. coronator and Cx. usquatus. Specimens were captured in Pariquera-Açu and Cananéia south-eastern São Paulo, Ribeira Valley, Brazil. Canonical variate analysis (CVA) demonstrated two groups in the morphospace. The accuracy of species recognition was moderate (82.6%) for Cx. coronator and low (60.8%) for Cx. usquatus. Bayesian analyses of concatenated gene sequences recovered from specimens of Cx. coronator separated the species into three lineages (herein referred to as Culex coronator A, B, and C), whereas Cx. usquatus specimens clustered into a single lineage. Lineages A and B of Cx. coronator intermixed with specimens of Cx. usquatus, and one specimen of Cx. coronator clustered into the Cx. usquatus lineage. The IMa analysis indicated that the divergence of Cx. coronator and Cx. usquatus is a slow process, with some degree of gene flow between the two species.

Original languageEnglish (US)
Pages (from-to)901-908
Number of pages8
JournalJournal of medical entomology
Volume54
Issue number4
DOIs
StatePublished - Jul 1 2017
Externally publishedYes

Keywords

  • geometric morphometrics
  • identification
  • species complex

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Wing morphometry and genetic variability between culex coronator and culex usquatus (Diptera: Culicidae), two sibling species of the coronator group'. Together they form a unique fingerprint.

Cite this