Wolbachia infections are virulent and inhibit the human malaria parasite Plasmodium falciparum in Anopheles gambiae

Grant L. Hughes, Ryuichi Koga, Ping Xue, Takema Fukatsu, Jason L. Rasgon

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Endosymbiotic Wolbachia bacteria are potent modulators of pathogen infection and transmission in multiple naturally and artificially infected insect species, including important vectors of human pathogens. Anopheles mosquitoes are naturally uninfected with Wolbachia, and stable artificial infections have not yet succeeded in this genus. Recent techniques have enabled establishment of somatic Wolbachia infections in Anopheles. Here, we characterize somatic infections of two diverse Wolbachia strains (wMelPop and wAlbB) in Anopheles gambiae, the major vector of human malaria. After infection, wMelPop disseminates widely in the mosquito, infecting the fat body, head, sensory organs and other tissues but is notably absent from the midgut and ovaries. Wolbachia initially induces the mosquito immune system, coincident with initial clearing of the infection, but then suppresses expression of immune genes, coincident with Wolbachia replication in the mosquito. Both wMelPop and wAlbB significantly inhibit Plasmodium falciparum oocyst levels in the mosquito midgut. Although not virulent in non-bloodfed mosquitoes, wMelPop exhibits a novel phenotype and is extremely virulent for approximately 12-24 hours post-bloodmeal, after which surviving mosquitoes exhibit similar mortality trajectories to control mosquitoes. The data suggest that if stable transinfections act in a similar manner to somatic infections, Wolbachia could potentially be used as part of a strategy to control the Anopheles mosquitoes that transmit malaria.

Original languageEnglish (US)
Article numbere1002043
JournalPLoS Pathogens
Volume7
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

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Wolbachia
Anopheles gambiae
Falciparum Malaria
Culicidae
Parasites
Anopheles
Infection
Mosquito Control
Infectious Disease Transmission
Malaria
Fat Body
Oocysts
Plasmodium falciparum
Insects
Ovary
Immune System
Head
Bacteria
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Wolbachia infections are virulent and inhibit the human malaria parasite Plasmodium falciparum in Anopheles gambiae. / Hughes, Grant L.; Koga, Ryuichi; Xue, Ping; Fukatsu, Takema; Rasgon, Jason L.

In: PLoS Pathogens, Vol. 7, No. 5, e1002043, 05.2011.

Research output: Contribution to journalArticle

Hughes, Grant L. ; Koga, Ryuichi ; Xue, Ping ; Fukatsu, Takema ; Rasgon, Jason L. / Wolbachia infections are virulent and inhibit the human malaria parasite Plasmodium falciparum in Anopheles gambiae. In: PLoS Pathogens. 2011 ; Vol. 7, No. 5.
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