X-linked, polymorphic genetic variation of thyroxin-binding globulin (TBG) in baboons and screening of additional primates

David H. Lockwood, Dorian H. Coppenhaver, Robert E. Ferrell, Stephen P. Daiger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

X-linked polymorphic variation of thyroxin-binding globulin (TBG) is observed in several human groups. Isoelectric focusing of plasma samples labeled in vitro with [125I]thyroxin, followed by autoradiography, also reveals genetically determined polymorphic electrophoretic variation in baboon TBG. The protein detected by this method in baboon plasma is immunologically similar to human TBG and is distinct from the other thyroxin-binding proteins, albumin and prealbumin. The isoelectric patterns of human and baboon TBG are very similar and both have an isoelectric range of pH 4.1 to 4.5. The baboon TBG polymorphism is inherited in a two-allele X-linked fashion, with a frequency of 72% for the "common" allele and 28% for the "slow" allele. A survey of seven other primate species including African green monkey, bonnet macaque, chimpanzee, crab-eating macaque, gorilla, rhesus monkey, and spider monkey revealed no polymorphic variation in TBG, although isoelectric patterns were similar to the human and baboon patterns. In addition, samples from pregnant chimpanzees demonstrate a pronounced quantitative anodal shift in relative band densities, a shift also observed in pregnant humans. This shift was not observed in samples from pregnant baboons. TBG should prove to be a useful X-linked genetic marker in baboons and provides a model of serum protein changes in pregnancy, at least in humans and chimpanzees.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalBiochemical Genetics
Volume22
Issue number1-2
DOIs
StatePublished - Feb 1984

Fingerprint

Papio
Globulins
thyroxine
Thyroxine
globulins
primate
Primates
genetic variation
allele
Screening
screening
protein
plasma
Pan troglodytes
genetic marker
pregnancy
spider
serum
crab
polymorphism

Keywords

  • baboons
  • pregnancy proteins
  • primate polymorphism
  • thyroxin-binding globulin (TBG)
  • X linkage

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

X-linked, polymorphic genetic variation of thyroxin-binding globulin (TBG) in baboons and screening of additional primates. / Lockwood, David H.; Coppenhaver, Dorian H.; Ferrell, Robert E.; Daiger, Stephen P.

In: Biochemical Genetics, Vol. 22, No. 1-2, 02.1984, p. 81-88.

Research output: Contribution to journalArticle

Lockwood, David H. ; Coppenhaver, Dorian H. ; Ferrell, Robert E. ; Daiger, Stephen P. / X-linked, polymorphic genetic variation of thyroxin-binding globulin (TBG) in baboons and screening of additional primates. In: Biochemical Genetics. 1984 ; Vol. 22, No. 1-2. pp. 81-88.
@article{598b54f692074d67833bdfd3a7f97a48,
title = "X-linked, polymorphic genetic variation of thyroxin-binding globulin (TBG) in baboons and screening of additional primates",
abstract = "X-linked polymorphic variation of thyroxin-binding globulin (TBG) is observed in several human groups. Isoelectric focusing of plasma samples labeled in vitro with [125I]thyroxin, followed by autoradiography, also reveals genetically determined polymorphic electrophoretic variation in baboon TBG. The protein detected by this method in baboon plasma is immunologically similar to human TBG and is distinct from the other thyroxin-binding proteins, albumin and prealbumin. The isoelectric patterns of human and baboon TBG are very similar and both have an isoelectric range of pH 4.1 to 4.5. The baboon TBG polymorphism is inherited in a two-allele X-linked fashion, with a frequency of 72{\%} for the {"}common{"} allele and 28{\%} for the {"}slow{"} allele. A survey of seven other primate species including African green monkey, bonnet macaque, chimpanzee, crab-eating macaque, gorilla, rhesus monkey, and spider monkey revealed no polymorphic variation in TBG, although isoelectric patterns were similar to the human and baboon patterns. In addition, samples from pregnant chimpanzees demonstrate a pronounced quantitative anodal shift in relative band densities, a shift also observed in pregnant humans. This shift was not observed in samples from pregnant baboons. TBG should prove to be a useful X-linked genetic marker in baboons and provides a model of serum protein changes in pregnancy, at least in humans and chimpanzees.",
keywords = "baboons, pregnancy proteins, primate polymorphism, thyroxin-binding globulin (TBG), X linkage",
author = "Lockwood, {David H.} and Coppenhaver, {Dorian H.} and Ferrell, {Robert E.} and Daiger, {Stephen P.}",
year = "1984",
month = "2",
doi = "10.1007/BF00499288",
language = "English (US)",
volume = "22",
pages = "81--88",
journal = "Biochemical Genetics",
issn = "0006-2928",
publisher = "Springer New York",
number = "1-2",

}

TY - JOUR

T1 - X-linked, polymorphic genetic variation of thyroxin-binding globulin (TBG) in baboons and screening of additional primates

AU - Lockwood, David H.

AU - Coppenhaver, Dorian H.

AU - Ferrell, Robert E.

AU - Daiger, Stephen P.

PY - 1984/2

Y1 - 1984/2

N2 - X-linked polymorphic variation of thyroxin-binding globulin (TBG) is observed in several human groups. Isoelectric focusing of plasma samples labeled in vitro with [125I]thyroxin, followed by autoradiography, also reveals genetically determined polymorphic electrophoretic variation in baboon TBG. The protein detected by this method in baboon plasma is immunologically similar to human TBG and is distinct from the other thyroxin-binding proteins, albumin and prealbumin. The isoelectric patterns of human and baboon TBG are very similar and both have an isoelectric range of pH 4.1 to 4.5. The baboon TBG polymorphism is inherited in a two-allele X-linked fashion, with a frequency of 72% for the "common" allele and 28% for the "slow" allele. A survey of seven other primate species including African green monkey, bonnet macaque, chimpanzee, crab-eating macaque, gorilla, rhesus monkey, and spider monkey revealed no polymorphic variation in TBG, although isoelectric patterns were similar to the human and baboon patterns. In addition, samples from pregnant chimpanzees demonstrate a pronounced quantitative anodal shift in relative band densities, a shift also observed in pregnant humans. This shift was not observed in samples from pregnant baboons. TBG should prove to be a useful X-linked genetic marker in baboons and provides a model of serum protein changes in pregnancy, at least in humans and chimpanzees.

AB - X-linked polymorphic variation of thyroxin-binding globulin (TBG) is observed in several human groups. Isoelectric focusing of plasma samples labeled in vitro with [125I]thyroxin, followed by autoradiography, also reveals genetically determined polymorphic electrophoretic variation in baboon TBG. The protein detected by this method in baboon plasma is immunologically similar to human TBG and is distinct from the other thyroxin-binding proteins, albumin and prealbumin. The isoelectric patterns of human and baboon TBG are very similar and both have an isoelectric range of pH 4.1 to 4.5. The baboon TBG polymorphism is inherited in a two-allele X-linked fashion, with a frequency of 72% for the "common" allele and 28% for the "slow" allele. A survey of seven other primate species including African green monkey, bonnet macaque, chimpanzee, crab-eating macaque, gorilla, rhesus monkey, and spider monkey revealed no polymorphic variation in TBG, although isoelectric patterns were similar to the human and baboon patterns. In addition, samples from pregnant chimpanzees demonstrate a pronounced quantitative anodal shift in relative band densities, a shift also observed in pregnant humans. This shift was not observed in samples from pregnant baboons. TBG should prove to be a useful X-linked genetic marker in baboons and provides a model of serum protein changes in pregnancy, at least in humans and chimpanzees.

KW - baboons

KW - pregnancy proteins

KW - primate polymorphism

KW - thyroxin-binding globulin (TBG)

KW - X linkage

UR - http://www.scopus.com/inward/record.url?scp=0021363383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021363383&partnerID=8YFLogxK

U2 - 10.1007/BF00499288

DO - 10.1007/BF00499288

M3 - Article

VL - 22

SP - 81

EP - 88

JO - Biochemical Genetics

JF - Biochemical Genetics

SN - 0006-2928

IS - 1-2

ER -