Xanthine oxidase mediates myocardial injury after hepatoenteric ischemia-reperfusion

Vance G. Nielsen, Sidhartha Tan, Manuel S. Baird, Paul N. Samuelson, Andrew T. McCammon, Dale A. Parks

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


Objectives: To determine if myocardial injury results from hepatoenteric ischemia-reperfusion. We also proposed to determine if this remote heart injury is mediated by a xanthine oxidase-dependent mechanism. Design: Randomized, controlled animal study. Setting: University-based animal research facility. Subjects: Thirty-six New Zealand white male rabbits, weighing 1.8 to 3 kg. Interventions: Anesthetized rabbits were randomly assigned to one of four groups (n = 9 per group): a) a sham-operated group; b) a sham-operated group pretreated with sodium tungstate (xanthine oxidase inactivator); c) an sorts occlusion group; and d) an aorta occlusion group pretreated with sodium tungstate. Descending thoracic aorta occlusion was maintained for 40 mins with a 4-Fr Fogarty embolectomy catheter, followed by 2 hrs of reperfusion. Measurements and Main Results: Myocardial injury, manifested by increased circulating creatine kinase-MB fraction activity, was significantly associated with aortic occlusion and reperfusion (p < .05). Sodium tungstate pretreatment significantly (p < .05) reduced circulating and myocardial xanthine oxidase activity. Xanthine oxidase inactivation by sodium tungstate significantly decreased circulating creatine kinase-MB fraction activity after hepatoenteric ischemia-reperfusion (p < .05). Finally, circulating creatine kinase-MB fraction activity was significantly associated with circulating xanthine oxidase activity (r2 = .85; p < .001). Conclusions: We conclude that remote myocardial injury is caused by hepatoenteric ischemia-reperfusion. The pathoetiology of this myocardial injury involves s xanthine oxidase-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)1044-1050
Number of pages7
JournalCritical care medicine
Issue number6
StatePublished - Jun 1997
Externally publishedYes


  • Critical care
  • Free radicals
  • Heart
  • Ischemia
  • Liver
  • Oxidants
  • Reperfusion
  • Shock
  • Sodium tungstate
  • Xanthine oxidoreductase

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine


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