Xenoestrogens at picomolar to nanomolar concentrations trigger membrane estrogen receptor-α-mediated Ca2+ fluxes and prolactin release in GH3/B6 pituitary tumor cells

Ann L. Wozniak, Nataliya N. Bulayeva, Cheryl S. Watson

    Research output: Contribution to journalArticle

    270 Citations (Scopus)

    Abstract

    Xenoestrogens (XEs) are widespread in our environment and are known to have deleterious effects in animal (and perhaps human) populations. Acting as inappropriate estrogens, XEs are thought to interfere with endogenous estrogens such as estradiol (E2) to disrupt normal estrogenic signaling. We investigated the effects of E2 versus several XEs representing organochlorine pesticides (dieldrin, endosulfan, o′,p′-dichlorodiphenylethylene), plastics manufacturing by-prodacts/detergents (nonylphenol, bisphenol A), a phytoestrogen (coumestrol), and a synthetic estrogen (diethylstilbestrol) on the pituitary tumor cell subline GH3/B6/F10, previously selected for expression of high levels of membrane estrogen receptor-α. Picomolar to nanomolar concentrations of both E2 and XEs caused intracellular Ca2+ changes within 30 sec of administration. Each XE produced a unique temporal pattern of Ca2+ elevation. Removing Ca2+ from the extracellular solution abolished both spontaneous and XE-induced intracellular Ca2+ changes, as did 10 μM nifedipine. This suggests that XEs mediate their actions via voltage-dependent L-type Ca2+ channels in the plasma membrane. None of the Ca2+ fluxes came from intracellular Ca2+ stores. E2 and each XE also caused unique time- and concentration-dependent patterns of prolactin (PRL) secretion that were largely complete within 3 min of administration. PRL secretion was also blocked by nifedipine, demonstrating a correlation between Ca2+ influx and PRL secretion. These data indicate that at very low concentrations, XEs mediate membrane-initiated intracellular Ca2+ increases resulting in PRL secretion via a mechanism similar to that for E2, but with distinct patterns and potencies that could explain their abilities to disrupt endocrine functions.

    Original languageEnglish (US)
    Pages (from-to)431-439
    Number of pages9
    JournalEnvironmental Health Perspectives
    Volume113
    Issue number4
    DOIs
    StatePublished - Apr 2005

    Fingerprint

    Pituitary Neoplasms
    tumor
    secretion
    Estrogen Receptors
    Prolactin
    Tumors
    Cells
    Fluxes
    membrane
    Membranes
    Nifedipine
    Estrogens
    Coumestrol
    Endosulfan
    Estradiol Congeners
    Dieldrin
    Phytoestrogens
    Intracellular Membranes
    Diethylstilbestrol
    dieldrin

    Keywords

    • Bisphenol A
    • Coumestrol
    • DDE
    • DES
    • Dieldrin
    • Diethylstilbestrol
    • Endosulfan
    • Estrogen receptor-α
    • Exocytosis
    • L-type channels
    • Membrane
    • Nonylphenol
    • Phytoestrogen
    • Prolactin
    • Xenoestrogen

    ASJC Scopus subject areas

    • Environmental Science(all)
    • Environmental Chemistry
    • Public Health, Environmental and Occupational Health

    Cite this

    Xenoestrogens at picomolar to nanomolar concentrations trigger membrane estrogen receptor-α-mediated Ca2+ fluxes and prolactin release in GH3/B6 pituitary tumor cells. / Wozniak, Ann L.; Bulayeva, Nataliya N.; Watson, Cheryl S.

    In: Environmental Health Perspectives, Vol. 113, No. 4, 04.2005, p. 431-439.

    Research output: Contribution to journalArticle

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    abstract = "Xenoestrogens (XEs) are widespread in our environment and are known to have deleterious effects in animal (and perhaps human) populations. Acting as inappropriate estrogens, XEs are thought to interfere with endogenous estrogens such as estradiol (E2) to disrupt normal estrogenic signaling. We investigated the effects of E2 versus several XEs representing organochlorine pesticides (dieldrin, endosulfan, o′,p′-dichlorodiphenylethylene), plastics manufacturing by-prodacts/detergents (nonylphenol, bisphenol A), a phytoestrogen (coumestrol), and a synthetic estrogen (diethylstilbestrol) on the pituitary tumor cell subline GH3/B6/F10, previously selected for expression of high levels of membrane estrogen receptor-α. Picomolar to nanomolar concentrations of both E2 and XEs caused intracellular Ca2+ changes within 30 sec of administration. Each XE produced a unique temporal pattern of Ca2+ elevation. Removing Ca2+ from the extracellular solution abolished both spontaneous and XE-induced intracellular Ca2+ changes, as did 10 μM nifedipine. This suggests that XEs mediate their actions via voltage-dependent L-type Ca2+ channels in the plasma membrane. None of the Ca2+ fluxes came from intracellular Ca2+ stores. E2 and each XE also caused unique time- and concentration-dependent patterns of prolactin (PRL) secretion that were largely complete within 3 min of administration. PRL secretion was also blocked by nifedipine, demonstrating a correlation between Ca2+ influx and PRL secretion. These data indicate that at very low concentrations, XEs mediate membrane-initiated intracellular Ca2+ increases resulting in PRL secretion via a mechanism similar to that for E2, but with distinct patterns and potencies that could explain their abilities to disrupt endocrine functions.",
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