XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability

Stephanie L. Moon, Jeffrey G. Blackinton, John R. Anderson, Mary K. Dozier, Benjamin J T Dodd, Jack D. Keene, Carol J. Wilusz, Shelton Bradrick, Jeffrey Wilusz

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We demonstrate that both Hepatitis C virus (HCV) and Bovine Viral Diarrhea virus (BVDV) contain regions in their 5’ UTRs that stall and repress the enzymatic activity of the cellular 5’-3’ exoribonuclease XRN1, resulting in dramatic changes in the stability of cellular mRNAs. We used biochemical assays, virus infections, and transfection of the HCV and BVDV 5’ untranslated regions in the absence of other viral gene products to directly demonstrate the existence and mechanism of this novel host-virus interaction. In the context of HCV infection, we observed globally increased stability of mRNAs resulting in significant increases in abundance of normally short-lived mRNAs encoding a variety of relevant oncogenes and angiogenesis factors. These findings suggest that non-coding regions from multiple genera of the Flaviviridae interfere with XRN1 and impact post-transcriptional processes, causing global dysregulation of cellular gene expression which may promote cell growth and pathogenesis.

Original languageEnglish (US)
Article numbere1004708
Pages (from-to)1-21
Number of pages21
JournalPLoS Pathogens
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Fingerprint

Bovine Viral Diarrhea Viruses
5' Untranslated Regions
RNA Stability
Hepacivirus
Virus Diseases
Flaviviridae
Angiogenesis Inducing Agents
Viral Proteins
Oncogenes
Transfection
Viruses
Gene Expression
Messenger RNA
Growth

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Moon, S. L., Blackinton, J. G., Anderson, J. R., Dozier, M. K., Dodd, B. J. T., Keene, J. D., ... Wilusz, J. (2015). XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability. PLoS Pathogens, 11(3), 1-21. [e1004708]. https://doi.org/10.1371/journal.ppat.1004708

XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability. / Moon, Stephanie L.; Blackinton, Jeffrey G.; Anderson, John R.; Dozier, Mary K.; Dodd, Benjamin J T; Keene, Jack D.; Wilusz, Carol J.; Bradrick, Shelton; Wilusz, Jeffrey.

In: PLoS Pathogens, Vol. 11, No. 3, e1004708, 01.03.2015, p. 1-21.

Research output: Contribution to journalArticle

Moon, SL, Blackinton, JG, Anderson, JR, Dozier, MK, Dodd, BJT, Keene, JD, Wilusz, CJ, Bradrick, S & Wilusz, J 2015, 'XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability', PLoS Pathogens, vol. 11, no. 3, e1004708, pp. 1-21. https://doi.org/10.1371/journal.ppat.1004708
Moon, Stephanie L. ; Blackinton, Jeffrey G. ; Anderson, John R. ; Dozier, Mary K. ; Dodd, Benjamin J T ; Keene, Jack D. ; Wilusz, Carol J. ; Bradrick, Shelton ; Wilusz, Jeffrey. / XRN1 Stalling in the 5’ UTR of Hepatitis C Virus and Bovine Viral Diarrhea Virus Is Associated with Dysregulated Host mRNA Stability. In: PLoS Pathogens. 2015 ; Vol. 11, No. 3. pp. 1-21.
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